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MetabolomicsPrediction of complex phenotypes using the Drosophila melanogaster metabolome
Understanding the genotype-phenotype map and how variation at different levels of biological organization is associated are central topics in modern biology. Fast developments in sequencing technologies and other molecular omic tools enable researchers to obtain detailed information on variation at ...
2021-08-05 | MTBLS2060 | MetaboLights
MetabolomicsSARS-CoV-2 ORF7a Drives Mitochondrial Dysfunction via PDK4 Activation and Complex I Inhibition

SARS-CoV-2 reprograms host metabolism to promote viral replication and evade immune responses. While infection is known to impair mitochondrial function and enhance glycolysis, the role of viral accessory proteins in these alterations remains unclear. Here, we investigate the metabolic impact of ...

2026-07-09 | MTBLS13338 | MetaboLights
ProteomicsProSight Native Complex Down Analysis of Aquaporin Z
In this dataset, we analyzed Aquaporin Z using native mass spectrometry. The data was then input into ProSight Native to determine the protein complex composition.
ORGANISM(S): Escherichia coli 
2023-08-14 | PXD038817 | Pride
Native mass spectrometry 
ProteomicsNative Prohibitin Complex Enrichment
Folding of newly synthesized proteins poses challenges for a functional proteome. Dedicated protein quality control (PQC) systems promote either the folding of nascent polypeptides at ribosomes or, if this fails, ensure their degradation. While well studied for cytosolic protein biogenesis, it is no...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-08-14 | PXD043671 | Pride
Yeast Immunoprecipitation Prohibitin Native complex 
ProteomicsQuantitative Native Proteomics by Capillary Zone Electrophoresis-Mass Spectrometry
Accurately measuring protein complex dynamics (i.e., composition and/or abundance changes) in cells is vital for advancing fundamental and translational research. In this work, we present a pilot study establishing capillary zone electrophoresis (CZE)-mass spectrometry (MS) based quantitative native...
ORGANISM(S): Escherichia coli 
2025-12-08 | PXD068962 | Pride
Capillary zone electrophoresis-mass spectrometry Quantitative native proteomics 
ProteomicsGlycine receptor complex analysis using immunoprecipitation-blue native gel electrophoresis-mass spectrometry
The pentameric glycine receptor (GlyR), comprising the α1 and β subunits, is a major inhibitory ionotropic receptor in brainstem and spinal cord. GlyRs interact with gephyrin (GPHN), a scaffold protein that anchors the GlyR in the plasma membrane and enables it to form clusters in glycinergic postsy...
ORGANISM(S): Mus musculus (Mouse) 
2022-12-12 | PXD017151 | Pride
Blue native gel electrophoresis Proteomics Protein complex Glycine receptor Iqsec3 
ProteomicsSelf-packed size-exclusion columns enable versatile high-throughput native, top-down, and ion-mobility-mass spectrometry studies on proteins and complexes.
Native MS (nMS) is a key structural biology technique that makes it possible to study intact proteins and their interactions. Unfortunately, non-volatile salts are incompatiblewith nMS, which demands a laborious desalting procedure. Non-denaturing size exclusion chromatography (SEC) allows both rapi...
ORGANISM(S): Bos taurus (Bovine) Candida albicans (Yeast) Homo sapiens (Human) Escherichia coli Gallus gallus (Chicken) Equus caballus (Horse) 
2025-06-29 | PXD061452 | Pride
Ligand screening Top-down proteomics Native mass spectrometry Size-exclusion chromatography Protac Ion mobility 
ProteomicsStructural inventory of native ribosomal ABCE1-43S pre-initiation complex
In eukaryotic translation, the termination and recycling phases are linked to subsequent initiation by persistence of several factors. These comprise the large eIF3 complex, eIF3j (Hcr1 in yeast) and the ATP-binding cassette protein ABCE1 (Rli1 in yeast). The ATPase is mainly active as a recycling f...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-10-16 | PXD020849 | Pride
Abce1 Ribosome Yeast Abc-proteins Translation initiation 
GenomicsProteomic analysis of native hepatocyte nuclear factor-4{alpha} (HNF4{alpha}) isoforms, phosphorylation status, and interactive cofactors.
Hepatocyte nuclear factor-4α (HNF4α, NR2A1) is a nuclear receptor which has a critical role in hepatocyte differentiation and the maintenance of homeostasis in the adult liver. However, a detailed understanding of native HNF4α in the steady state remains to be elucidated. Here we report the native H...
ORGANISM(S): Homo sapiens 
2010-12-22 | E-GEOD-18990 | biostudies-arrayexpress
ProteomicsMulti-dimensional native proteomics technology for protein complex identification and topology characterization (Mud-NatPIT)
Here, we demonstrate a pilot development of multi-dimensional native proteomics technology for protein complex identification and topology characterization (Mud-NatPIT) in complex biological samples via coupling native size exclusion chromatography (SEC), online native capillary electrophoresis-mass...
ORGANISM(S): Escherichia coli 
2026-08-04 | PXD069439 | Pride
Capillary electrophoresis-mass spectrometry Protein complex identification Native proteomics Topology characterization Surface-induced dissociation 
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