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MetabolomicsBiocontrol potential and antifungal mechanism of three native endophytic Bacillus

Chinese yam tubers are nutrient-rich but highly susceptible to mechanical damage during harvest, which facilitates pathogenic fungal infection at wound sites. This study aimed to identify pathogenic fungi from postharvest yam wounds and screen native endophytic bacteria with antifungal activity f...

2026-10-06 | MTBLS14657 | MetaboLights
ProteomicsApplication of liquid-liquid extraction for N-terminal myristoylation proteomics
Proteins can be modified by lipids in various ways, for example by myristoylation, palmitoylation, farnesylation, and geranylgeranylation—these processes are collectively referred to as lipidation. Current chemical proteomics using alkyne lipids has enabled the identification of lipidated protein ca...
ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse) 
2023-11-09 | PXD041499 | JPOST Repository
TranscriptomicsNative versus cultured retinal pigment epithelium cells
I. Exp Design 1. Type of experiment: Comparison of native versus cultured RPE cells 2. Experimental factors: Native RPE versus ARPE-19 cells grown on different matrices 3. How many hybridizations in exp: 21 4. If a common reference used for all the hybs: no 5. Quality control steps: three independen...
ORGANISM(S): Homo sapiens 
2010-06-05 | E-GEOD-741 | biostudies-arrayexpress
ProteomicsActivity guided profiling of yeast proteasome - protein extraction test
This experiment compared the impact of protein extraction conditions on the identification and quantification of proteasome subunits in WT yeast. It involved comparing proteins extracted from identical WT yeast samples under two different conditions: native and denaturing (in the presence of SDS and...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-02-15 | PXD048380 | Pride
Native extraction Proteasome Yeast lfq 
ProteomicsA new method for differential proteomic analysis utilizing native MS-electropherograms: a comparison between plasma and serum proteins by combining native PAGE, whole-gel slicing and quantitative LC-MS/MS
MS identification has long been used for PAGE-separated protein bands, but global and systematic quantitation utilizing MS after PAGE has remained rare and not been reported for native PAGE. Here we reported on a new method combining native PAGE, whole-gel slicing and quantitative LC-MS/MS aiming c...
ORGANISM(S): Homo sapiens (Human) 
2017-09-08 | PXD006773 | Pride
Gel slicing Quantitative lc-ms/ms Native ms-electropherogram Native 1d-page Human plasma and serum Differential proteomic analysis 
TranscriptomicsTranscription profiling of human native odontoblasts and pulp tissue
Knowledge of differential gene expression between pulp and odontoblasts might give insight to the regulation of these spatially related but functionally diverse cells. Our aim was large-scale analysis of expression profiles of native human pulp tissue and odontoblasts, and search for genes expressed...
ORGANISM(S): Homo sapiens 
2008-06-16 | E-GEOD-8694 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human native skin, cultured skin substitute, constituent keratinocytes and fibroblasts to identify gene signatures that are highly characteristic of different tissue types
Cultured skin substitutes, prepared using keratinocytes, fibroblasts and biopolymers, can facilitate closure of massive burn wounds by increasing the availability of autologous tissue for grafting. However, because they contain only two cell types, skin substitutes cannot replace all of the functio...
ORGANISM(S): Homo sapiens 
2007-09-06 | E-GEOD-3204 | biostudies-arrayexpress
UnknownProbing the Fundamentals of Native Liquid Extraction Surface Analysis Mass Spectrometry of Proteins: Can Proteins Refold during Extraction?
Not available
S-EPMC7006963 | biostudies-literature
TranscriptomicsGenomic expression study of regenerated endothelial cells in high cholesterol-fed and fish oil-rich groups
Experiments on primary cultures of native and regenerated endothelial cells demonstrated genomic changes in the latter related to vasomotor control, coagulation, oxidative stress, lipid metabolism and extracellular matrix. However, the genomic changes caused by the combination of either hyperlipidem...
ORGANISM(S): Sus scrofa 
2010-10-18 | E-GEOD-24762 | biostudies-arrayexpress
GenomicsHigh-resolution mapping of transcription factor binding sites on native chromatin
Sequence-specific DNA-binding proteins including transcription factors (TFs) are key determinants of gene regulation and chromatin architecture. Formaldehyde cross-linking and sonication followed by Chromatin ImmunoPrecipitation (X-ChIP) and sequencing is widely used for genome-wide profiling of pro...
ORGANISM(S): Drosophila melanogaster 
2013-12-14 | E-GEOD-45672 | biostudies-arrayexpress
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