Sort   by:  
 Page size 
ProteomicsMS-based determination of NELF-A phosphorylation
LC-MS/MS analysis of Flag-NELF-A purified from TPA-stimulated HEK293 cells
ORGANISM(S): Homo Sapiens (human) 
2022-11-15 | PXD038094 | JPOST Repository
ProteomicsNELF-A phosphorylation site identification
NELF-A phosphorylation by P-TEFb is a key event in the pausing release. We conduct IMAC-LC-MS-MS to identified NELF-A phosphorylation site by activation P-TEFb kinase.
ORGANISM(S): Homo sapiens (Human) 
2016-03-23 | PXD003433 | Pride
Imac Nelf-a Hela cell Human 
GenomicsRegulation of Mammalian Cell Growth and Survival By an RNA Polymerase II-Pausing Factor (ChIP-seq)
Many mammalian genes are occupied by paused RNA polymerase II (pol II) at promoter-proximal regions on both sides of transcription start sites (TSSs). However, the consequences of pol II pausing on gene expression and cell biology are not fully understood. Here we report that genetic ablation of t...
ORGANISM(S): Mus musculus 
2011-08-17 | E-GEOD-24113 | biostudies-arrayexpress
GenomicsIntegrator complex regulates NELF-mediated RNA Polymerase II pause/release and processivity at coding genes [ChIP-seq]
RNAPII pausing/termination shortly after initiation is a hallmark of gene regulation. However, the molecular mechanisms involved are still to be uncovered. Here, we show that NELF interacts with Integrator complex subunits (INTScom) forming a stable complex with RNPII and Spt5. The interaction betwe...
ORGANISM(S): Homo sapiens 
2015-05-19 | E-GEOD-60584 | biostudies-arrayexpress
GenomicsNELF-dependent transcription activation
Jurkat T-cells were latently infected with a defective HIV provirus carrying a GFP reporer gene. The cells were superinfected with a lentiviral vector expressing shRNA to NELF-E. The impact of NELF-depletion on RNAP II distribution on cellular genes and the HIV provirus was measured by ChIP-Seq. D...
ORGANISM(S): Homo sapiens 
2014-03-26 | E-GEOD-47481 | biostudies-arrayexpress
ProteomicsDependency of NELF-E-KAT2B epigenetic axis in breast cancer carcinogenesis
Cancer cells undergo transcriptional reprogramming to drive tumor progression and metastasis. Here, we identified the transcriptional complex, NELF (Negative elongation factor), as an important regulator of this process. Using cancer cell lines and patient-derived tumor organoids, we demonstrated th...
ORGANISM(S): Homo Sapiens (human) 
2023-01-28 | PXD031304 | JPOST Repository
TranscriptomicsTranslation initiation at a non-AUG start codon for mouse and human NELF-B
Negative elongation factor (NELF), a four-subunit protein complex in metazoan, plays an important role in regulating promoter-proximal pausing of RNA polymerase II (RNAPII). Genetic studies demonstrate that the B subunit of mouse NELF (NELF-B) is critical for embryonic development and homeostasis i...
ORGANISM(S): Mus musculus 
2014-10-11 | E-GEOD-62248 | biostudies-arrayexpress
TranscriptomicsIntegrator complex regulates NELF-mediated RNA Polymerase II pause/release and processivity at coding genes [RNA-seq]
RNAPII pausing/termination shortly after initiation is a hallmark of gene regulation. However, the molecular mechanisms involved are still to be uncovered. Here, we show that NELF interacts with Integrator complex subunits (INTScom) forming a stable complex with RNPII and Spt5. The interaction betwe...
ORGANISM(S): Homo sapiens 
2015-05-19 | E-GEOD-60585 | biostudies-arrayexpress
GenomicsNELF-A controls Drosophila healthspan by regulating heat-shock protein-mediated cellular protection and heterochromatin maintenance
NELF-A controls Drosophila healthspan by regulating heat-shock protein-mediated cellular protection and heterochromatin maintenance
PRJNA656115 | ENA
UnknownNELF is a nuclear protein involved in hypothalamic GnRH neuronal migration.
Not available
S-EPMC3437992 | biostudies-literature
Sort   by:  
 Page size