The Pierce™ Magnetic RNA–Protein Pull-Down Kit (Thermo Fisher Scientific) was used to examine proteins associated with Ppp1r1b-lncRNA. The assay was performed using C2C12 cell lysates prepared from both undifferentiated and differentiated states. Full-length in vitro–transcribed Ppp1r1b-lncRNA was u...
Purpose: The growth and survival of NF2-deficient cells are enhanced by the activation of multiple signaling pathways including ErbB2/IGF-1R/Met, PI3K/Akt, and Ras/Raf/Mek/Erk1/2. The ubiquitously expressed chaperone protein HSP90 is known to be essential for the stabilization of these signaling mol...
Comprehensive knowledge of the genomic alterations that underlie cancer is a critical foundation for diagnostics, prognostics and targeted therapeutics. Systematic efforts to analyze cancer genomes are underway, but the analysis is hampered by the lack of a statistical framework to distinguish mean...
Integrity of the blood-brain barrier (BBB) is critical for brain homeostasis, and its malfunction contributes to neurovascular and neurodegenerative disorders. So far, mechanistic studies on BBB function have been mostly conducted in rodent and non-physiological in vitro models, which recapitulate s...
FOXF2, a major risk gene for stroke and SVD, the mechanisms of which are insufficiently understood. CRISPR/Cas9-mediated knockout of FOXF2 induced key features of BBB dysfunction, including compromised cell junction integrity, decreased transendothelial electrical resistance and dysregulated caveola...
Function and integrity of the blood-brain-barrier (BBB) is crucial for brain homeostasis, and its malfunction critically contributes to neurovascular and neurodegenerative disorders, including cerebral small vessel disease (SVD), a common cause of stroke and vascular dementia. So far, mechanistic st...
