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We found that LC3B is phosphorylated in vitro by STK3, STK4, NEK9, and PKCζ. To identify phosphorylation sites following in vitro phosphorylation, GST-LC3B was overexpressed in E. coli, purified using glutathione-Sepharose 4 Fast Flow beads and incubated in kinase assays with recombinant kinases or ...
ORGANISM(S): Homo sapiens (Human) 
2020-01-23 | PXD016681 | Pride
Co-immunoprecipitation was performed using the cell lysate after mitochondrial isolation, and then the proteins related to NIMA-related kinase 7 were detected by LC-MS/MS. For the mass spectrometry, Q-Exactive HF X(Thermo Fisher) was used. All the proteins are determined by matching the peptides ...
ORGANISM(S): Mus Musculus 
2025-09-24 | PXD068758 |
NIMA Related Kinase 7 reduction mediates acetaminophen-induced acute liver injury: role of impaired cell cycle regulation
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