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TranscriptomicsAcyclic retinoid targets platelet-derived growth factor signaling in the prevention of hepatic fibrosis and hepatocellular carcinoma development
Gene expression profiling in non tumor lesion in the liver of patients prior to administering peretinoin was unrelated to the clinical outcome, however, gene expression profiling 8 weeks after starting peretinoin therapy significantly predicted the recurrence of HCC Peretinoin, a member of the acycl...
ORGANISM(S): Homo sapiens 
2012-08-19 | E-GEOD-29302 | biostudies-arrayexpress
ProteomicsLFQ of biotinylated peptides from HEK293T cells expressing AirID-CRBN
HEK293T cells stably expressing AGIA-AirID-CRBN-WT were treated with DMSO, 20 µM thalidomide, 10 µM lenalidomide, 10 µM pomalidomide, or 20 µM 5-hydroxythalidomide (5HT) in the presence of 10 µM biotin and 5 µM MG132 for 8 h in three biological replicates. After tryptic digestion, biotinylated pepti...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028756 | JPOST Repository
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing HKDC1-mNeonGreen
Cells stably expressing full-length HKDC1-mNeonGreen or △N20 HKDC1-mNeonGreen proteins were grown in a 10-cm dish and were treated with or without antimycin/oligomycin for 3h, and then cross-linked with 0.1% formaldehyde for 10min at room temperature. After cross-linking was quenched with 100mM glyc...
ORGANISM(S): Homo Sapiens (human) 
2024-01-21 | PXD040970 | JPOST Repository
ProteomicsPrimPol-phosphoproteomics by IP-GeLC-MS/MS
Myc-tagged PrimPol expressed in HEK-293T cells was immunoprecipitated and separated by SDS-PAGE. Protein bands were excised and subjected to in-gel tryptic digestion and phosphopeptide enrichment by Fe-IMAC.
ORGANISM(S): Homo Sapiens (human) 
2023-08-22 | PXD043419 | JPOST Repository
ProteomicsLFQ of GFP-Trap IP-MS from MEF cells expressing GFP-WIPI2d
Control or GFP-WIPI2d expressing MEFs were treated with or without 0.5uM Apilimod for one hour, and then cross-linked with 0.1% formaldehyde for ten mins at room temperature. After cross-linking was quenched with 100mM glycine for four mins at room temperature, cells were washed with HEPES saline co...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-25 | PXD043701 | JPOST Repository
ProteomicsLFQ of GFP-Trap IP-MS from MEF cells expressing GFP-Rubicon
Control or GFP-Rubicon expressing MEFs were treated with or without 0.5uM Apilimod for one hour, and then cross-linked with 0.1% formaldehyde for ten mins at room temperature. After cross-linking was quenched with 100mM glycine for four mins at room temperature, cells were washed with HEPES saline c...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-25 | PXD043695 | JPOST Repository
ProteomicsTMT-based proteomic analysis of Atg5-/- mouse brain
The brains of neonatal mice (Atg5+/+ in triplicate, Atg5-/- in quadruplicate, and Atg5-/-;NSE-Atg5 in triplicate) were lysed in 500 µL of 6 M guanidine-HCl containing 100 mM Tris-HCl, pH8.0, and 2 mM DTT. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 g fo...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-01 | PXD049220 | JPOST Repository
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing TFEB-mNeonGreen
Cells stably expressing TFEB-mNeonGreen proteins were grown in a 10 cm dish and treated with or without LLOMe for 1 h or 3 h, then cross-linked with 0.1% formaldehyde for 10 min at room temperature. After cross-linking was quenched with 100 mM glycine for 4 min at room temperature, cells were washed...
ORGANISM(S): Homo Sapiens (human) 
2025-08-17 | PXD043288 | JPOST Repository
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing TFEB-mNeonGreen treated with MK6-83
Cells stably expressing TFEB-mNeonGreen proteins were grown in a 10 cm dish and treated with or without MK6-83 for 3 h, then cross-linked with 0.1% formaldehyde for 10 min at room temperature. After cross-linking was quenched with 100 mM glycine for 4 min at room temperature, cells were washed with ...
ORGANISM(S): Homo Sapiens (human) 
2025-08-17 | PXD053327 | JPOST Repository
ProteomicsLFQ of indisulam-dependent biotinylated peptides from HCT116 cells expressing AirID-DCAF15
HCT116 cells stably expressing AGIA-AirID-DCAF15-WT or -D475N mutant were treated with DMSO or 5 µM indisulam in the presence of 50 µM biotin and 10 µM MG132 for 6 h in three biological replicates. After tryptic digestion, biotinylated peptides were enriched with Tamavidin 2-REV and analyzed by LC-M...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028762 | JPOST Repository
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