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ProteomicsMetabolic labeling of NMT substrates by photoactivatable/clickable myristate probes X3/X8/X10
Efficiency of labelling by photoactivatable/clickable myristate probes X3/X8/X10 is compared to the positive control probe YnMyr. Additionally, negative controls for each probe were obtained by treatment with NMT ihibitor IMP-366 (DDD85464). The resulting data indicates efficient labeling of NMT sub...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD027239 | Pride
Nmt Diazirine Metabolic labeling Myristoylation Photocrosslinking 
GenomicsNMT inhibition in the context of MYC levels
NMT inhibition in the context of MYC levels
PRJNA645908 | ENA
ProteomicsN-Myristoyltransferase inhibition in HeLa
N-Myristoyltransferase (NMT) covalently attaches a C14-fatty acid to the N-terminal glycine of proteins and has been proposed as a therapeutic target in cancer. We used quantitative proteomics to map protein expression changes for more than 2700 proteins in response to treatment with an NMT inhibito...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080783 | MassIVE
UnknownStructure of Plasmodium vivaxN-myristoyltransferase with inhibitor IMP-1088: exploring an NMT inhibitor for antimalarial therapy.
Not available
S-EPMC11701927 | biostudies-literature
ProteomicsLabel-free quantification of NMT1 and NMT2 knockout as well as PCLX-001 treated HAP1 cells
HAP1 cells with an NMT1, NMT2, or double knockout (as well as wildtype controls) were prepared by in-gel trypsin digestion and analyzed by LC-MS/MS label-free quantification (data-dependent acquisition). Additionally, HAP1 cells were treated with PCLX-001, an NMT inhibitor, at EC50 and EC90 (as well...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-05-10 | MSV000091913 | MassIVE
ProteomicsIdentification of protein-protein interaction mediated by N-myristoylated proteins using photocrosslinkable clickable myristoylation probes X3 and X10
HeLa cells were treated with myristoylation probes X3 and X10, which incorporate both alkyne and diazirine moieties. Cells were then treated with NMT ihibitor IMP-366 (DDD85464) or DMSO vehicle and UV-irradiated before lysis. The experiment identifies myristoylated proteins through comparison of veh...
ORGANISM(S): Homo sapiens (Human) 
2023-02-04 | PXD029944 | Pride
Diazirine Nmt Photocrosslinking Myristoylaion 
ProteomicsProfiling N-myristoyltransferase activity and inhibition in breast cancer cells (MD-MB-231) using Sortase-A labelling
We report a novel application for Sortase A (SrtA), labeling N-terminal Gly proteins across the proteome at endogenous levels. We combined SrtA labeling of whole-cell lysates with a potent and selective NMT inhibitor, delivering a method capable of quantifying changes in cellular NMT activity in mul...
ORGANISM(S): Homo sapiens (Human) 
2018-10-25 | PXD010634 | Pride
Sortase a  srta  n-myristoylation  nmt 
GenomicsEffect of NMT inhibition on a model of NALFD in mice
Effect of NMT inhibition on a model of NALFD in mice
PRJNA929512 | ENA
TranscriptomicsEffect of NMT inhibition on a model of NALFD in mice
To examine the effects of NMTi treatment on progression of NAFLD model, mice on chow or high-fat diet were treated with vehicle or additionally on high-fat diet treated with DDD86481. Transcriptional analysis was performed after 19 weeks of high-fat diet with monthly treatments with 3 rounds of NMT ...
ORGANISM(S): Mus musculus 
2023-09-28 | GSE224069 | GEO
TranscriptomicsRNA-seq analysis of the overexpression of the Fil1 transcription factor in S. pombe
The Fil1 transcription factor regulates the response to amino acid starvation. Here we analyse the effects of overexpressing the fil1 coding sequence and of its deregulated expression. The following strains were used: 1] Strains expressing the fil1 gene under the control of the thiamine-repressib...
ORGANISM(S): Schizosaccharomyces pombe 
2018-02-01 | E-MTAB-6226 | biostudies-arrayexpress
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