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ProteomicsDevelopmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium
The clearance of untranslated mRNAs by Argonaute proteins is essential for embryonic development in metazoans. However, it is currently unknown whether a similar process exists in unicellular eukaryotes. The ciliate Paramecium tetraurelia harbors a vast array of Argonaute proteins of the Piwi-clade ...
ORGANISM(S): Paramecium tetraurelia 
2023-05-10 | PXD038984 | Pride
Paramecium tetraurelia Piwi Mrna clearance Untranslated mrna Total protein < 25kda Sirnas 
ProteomicsRNA-mediated nucleosome depletion is required for elimination of transposon-derived DNA
To study the role of chromatin remodeler during programmed genome reorganization in Paramecium tetraurelia. Paramecium ISWI1 was tagged with 3 FLAG and HA at its C-terminal. The recombinant plasmid was transformed into Paramecium and used for co-immunoprecipitation and Mass spectrometry studies to i...
ORGANISM(S): Paramecium tetraurelia 
2023-03-11 | PXD027206 | Pride
Paramecium tetraurelia Transposable elements Genome reorganization Nucleosome positioning Epigenetics project Iswi Chromatin 
ProteomicsIdentification of the Polycomb repressive complex 2 and its interacting proteins in Paramecium tetraurelia
Please provide an overall description of your study, think something similar in scope to the manuscript abstractn Paramecium tetraurelia, Ezl1 (Homolog of EZH2) and PtCAF1 (homolog of RbAp46/48) play important roles in genome rearrangement by disrupting the transposon and transposon-derived DNA elim...
ORGANISM(S): Paramecium tetraurelia 
2023-05-10 | PXD028503 | Pride
Polycomb repressive complex 2 Paramecium tetraurelia Immunoprecipitation 
ProteomicsStructural maintenance of chromosomes (SMCs) proteins are required for developmental DNA elimination in Paramecium tetraurelia
To study the role of structural maintenance of chromosome (SMC)4-1 and SMC4-2 during programmed genome rearrangement in Paramecium tetraurelia. Paramecium SMC4-1 and SMC4-2 was tagged with 3 FLAG and HA at its C-terminal separately. The recombinant plasmid was microinjected into macronuclear and use...
ORGANISM(S): Paramecium tetraurelia 
2023-12-05 | PXD041826 | Pride
Paramecium tetraurelia Dna elimination Mitosis Structural maintenance of chromosome (smc) Meiosis 
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