Sort   by:  
 Page size 
MetabolomicsTFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis
The mechanisms underlying cancer metastasis remain poorly understood. Here, we report that TFAM deficiency rapidly and stably induced spontaneous lung metastasis in mice with liver cancer. Interestingly, unexpected polymerization of nuclear actin was observed in TFAM-knockdown HCC cells when cytoske...
2022-06-23 | MTBLS4307 | MetaboLights
ProteomicsProteomics analysis of nuclear envelope, cytoplasmic membrane and nuclear contents fractions isolated from mesenchymal stem cells, adipocytes and myocytes
We used shotgun proteomics to analyze three subcellular fractions isolated from mesenchymal stem cells and from differentiated adipocytes and myocytes: nuclear envelopes (NEs), cytoplasmic membranes and nuclear contents. The proteomics data were analyzed by a NSAF-based scoring system to calculate...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-11-27 | MSV000083166 | MassIVE
TranscriptomicsRNAi knock down of dU2AF50 in Drosophila mel2 cells and profiling of nuclear and cytoplasmic fractions
Nuclear and cytoplasmic RNA were extracted by hypotonic cell lysis from D.-mel2 cell knock down by RNAi against dU2AF50 or with non-specific dsRNA.
ORGANISM(S): Drosophila melanogaster 
2007-07-05 | E-GEOD-1344 | biostudies-arrayexpress
TranscriptomicsDeep Sequencing of Human Nuclear and Cytoplasmic Small RNAs Reveals an Unexpectedly Complex Subcellular Distribution of miRNAs and tRNA 3' Trailers
Background: MicroRNAs (miRNAs) are 22-nt small non-coding regulatory RNAs that have generally been considered to regulate gene expression at the post-transcriptional level in the cytoplasm. However, recent studies have reported that some miRNAs localize to and function in the nucleus. Methodology/Pr...
ORGANISM(S): Homo sapiens 
2010-07-14 | E-GEOD-22918 | biostudies-arrayexpress
TranscriptomicsKnockdown of SR protein family in P19 cells and RNA-seq of cytoplasmic and whole cell polyA+ RNA
Our data suggest that all SR proteins contribute to mRNA export via NXF1. To identify endogenous export targets we depleted all seven SR proteins individually from P19 WT cells prepared cytoplasmic fractions. We sequenced the cytoplasmic fraction and as a control whole celll RNA from the identical s...
ORGANISM(S): Mus musculus 
2016-03-07 | E-GEOD-69733 | biostudies-arrayexpress
TranscriptomicsEfficient cellular fractionation of RNA
The aim of this experiment was to develop new methods to extract pure fractions of nuclear and cytoplasmic RNA. We compared the results of nuclear and cytoplasmic RNA-sequencing to results of the total and polyA+ RNA-sequencing. Cytoplasmic, nuclear, total and polyA+ RNA was extracted from two human...
ORGANISM(S): Homo sapiens 
2013-10-22 | E-MTAB-1898 | biostudies-arrayexpress
ProteomicsEnhanced interactome capture (eRIC) of steady-state and arsenite stressed HuH7 cell cytoplasmic and nuclear fractions
HuH7 cells were grown under steady-state conditions or exposed 100 µM Na-arsenite for 1h. Subsequently, the cells were irradiated or not with 253 nm UV light (150 mJ/cm^2), processed to nuclear and cytoplasmic fractions, and subjected to eRIC for determining the nuclear and cytoplasmic RNA-binding ...
ORGANISM(S): Homo sapiens (Human) 
2020-04-06 | PXD016735 | Pride
Human Cell culture Arsenite 
ProteomicsNucleo-cytoplasmic fractionation MS for proteins mislocalized with cytoplasmic TDP-43 in human iPSC-derived neurons
We aimed to unbiasedly identify proteins mislocalizing with cytoplasmic TDP-43 from the nucleus to the cytoplasm. We therefore expressed TDP-43deltaNLS or nuclear export signal (NES) as cytoplasmic control in induced neurons and performed cellular fractionation, separating nuclear from cytoplasmic f...
ORGANISM(S): Homo sapiens (Human) 
2026-10-15 | PXD063188 | Pride
Lc-ms Ipsc-derived neurons Human 
Genomics3' sequencing of nuclear and cytoplasmic fractions following treatment with a CLK inhibitor
3' sequencing of nuclear and cytoplasmic fractions following treatment with a CLK inhibitor
PRJEB65860 | ENA
Genomics3' sequencing of nuclear and cytoplasmic fractions following expression of an R-MCD
3' sequencing of nuclear and cytoplasmic fractions following expression of an R-MCD
PRJEB65473 | ENA
Sort   by:  
 Page size