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GenomicsInteraction between TBP and condensing drives the organization and faithful segregation of mitotic chromosomes
Genome/chromosome organization is highly ordered and controls nuclear events. Here, we show that the TATA box-binding protein (TBP) interacts with the Cnd2 kleisin subunit of condensin to mediate interphase and mitotic chromosome organization in fission yeast. TBP recruits condensin onto RNA polymer...
ORGANISM(S): Schizosaccharomyces pombe 
2015-08-12 | E-GEOD-60273 | biostudies-arrayexpress
GenomicsRole of Fft3 in nuclear organization [MNase-seq]
In eukaryotic cells, local chromatin structure and chromatin organization in the nucleus both influence transcriptional regulation. At the local level, the Fun30 chromatin remodeler Fft3 is essential for maintaining proper chromatin structure at centromeres and subtelomeres in fission yeast. Using g...
ORGANISM(S): Schizosaccharomyces pombe 
2015-03-02 | E-GEOD-58012 | biostudies-arrayexpress
ProteomicsSubfractionation of Nuclear Matrix (NuMat)
NuMat is a residual structure resistant to solubilization by nucleases, detergents, and salt solutions that retains the morphology of the nucleus. Purported to be the basis of nuclear architecture, it has remained further undissected. We use a chaotrope to subfractionate the nuclear matrix and decip...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-10-25 | PXD031296 | Pride
Nuclear organization Nuclear matrix Drosophila melanogaster 
TranscriptomicsRole of Fft3 in nuclear organization [DamID-chip, ChIP-chip]
In eukaryotic cells, local chromatin structure and chromatin organization in the nucleus both influence transcriptional regulation. At the local level, the Fun30 chromatin remodeler Fft3 is essential for maintaining proper chromatin structure at centromeres and subtelomeres in fission yeast. Using g...
ORGANISM(S): Schizosaccharomyces pombe 
2015-03-02 | E-GEOD-47464 | biostudies-arrayexpress
TranscriptomicsThe insulator protein SU(HW) modulates nuclear lamina interactions of the Drosophila genome: DamID
Specific interactions of the genome with the nuclear lamina (NL) are thought to assist chromosome folding inside the nucleus and to contribute to the regulation of gene expression. High-resolution mapping has recently identified hundreds of large, sharply defined lamina-associated domains (LADs) in ...
ORGANISM(S): Drosophila melanogaster 
2011-01-24 | E-GEOD-20311 | biostudies-arrayexpress
TranscriptomicsNuclear scaffold and matrix attachment of Aortic Adventitial Fibroblast (AoAF) chromatin
The nuclear scaffold/matrix provides an anchor for higher order genome structure that has both structural and functional implications. Different extraction protocols, i.e., utilizing either 25 mM LIS or 2 M NaCl, isolate somewhat different protein constituents of either the nuclear scaffold or nucl...
ORGANISM(S): Homo sapiens 
2009-03-10 | E-GEOD-13792 | biostudies-arrayexpress
ProteomicsLEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation
Quantitative crosslinking analysis to probe in vitro conformational dynamics governing ESCRT-mediated nuclear envelope formation. CHMP7 was crosslinked with D12-BS3 while CHMP7 in the presence of LEM2 winged helix domain was crosslinked with H12-BS3. Samples were mixed after crosslinking and analy...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-01-24 | MSV000084837 | MassIVE
TranscriptomicsGlobal chromatin domain organization of the Drosophila genome
In eukaryotes, neighboring genes can be packaged together in specific chromatin structures that ensure their coordinated expression. Examples of such multi-gene chromatin domains are well-documented, but a global view of the chromatin organization of eukaryotic genomes is lacking. To systematically ...
ORGANISM(S): Drosophila melanogaster 
2008-04-07 | E-GEOD-10219 | biostudies-arrayexpress
TranscriptomicsChromosomal translocations are guided by the spatial organization of the genome
The extent to which the three-dimensional organization of the genome contributes to chromosomal translocations is an important question in cancer genomics. We now have generated a high-resolution Hi-C spatial organization map of the G1-arrested mouse pro-B cell genome and mapped translocations from...
ORGANISM(S): Mus musculus 
2012-02-16 | E-GEOD-35519 | biostudies-arrayexpress
ProteomicsComparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelope
The nuclear envelope (NE) is a subdomain of the ER with a distinctive protein composition that underpins its role in nuclear compartmentalization. To expand an understanding of NE functions, we refined methods to reveal low abundance transmembrane (TM) proteins concentrated at the NE. De facto, th...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-01-25 | MSV000091154 | MassIVE
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