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TranscriptomicsInducible repositioning of mouse gene tethering to the inner nuclear membrane
Nuclear compartmentalization appears to play an important role in regulating metazoan genes. While studies on immunoglobulin (Ig) and other loci have correlated positioning at the nuclear lamina with gene repression, the functional consequences of this compartmentalization remain untested. We devise...
ORGANISM(S): Mus musculus 
2008-06-11 | E-GEOD-10176 | biostudies-arrayexpress
TranscriptomicsChiP-chip profiling of Drosophila melanogaster cell lines to identify targets for nuclear pore proteins Nup153 and Megator.
ChIP-chip experiment for nuclear pore proteins Nup153 and Mtor in Drosophila S2 and Kc cells. This experiment is related to E-MEXP-2523.
ORGANISM(S): Drosophila melanogaster 
2010-02-12 | E-MEXP-2525 | biostudies-arrayexpress
ProteomicsProteomics analysis of nuclear envelope, cytoplasmic membrane and nuclear contents fractions isolated from mesenchymal stem cells, adipocytes and myocytes
We used shotgun proteomics to analyze three subcellular fractions isolated from mesenchymal stem cells and from differentiated adipocytes and myocytes: nuclear envelopes (NEs), cytoplasmic membranes and nuclear contents. The proteomics data were analyzed by a NSAF-based scoring system to calculate...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-11-27 | MSV000083166 | MassIVE
TranscriptomicsIdentification of the mRNAs interacting with the Nup59 nucleoporin using RNA-IP and microarrays in budding yeast
To specifically explore such mRNA localization events, we immunopurified the entire Nuclear Pore Complex scaffold using Nup59 as a bait and systematically analyzed all co-isolating mRNAs using Agilent microarrays in the budding yeast Saccharomyces cerevisiae. A mock IP was performed as a control exp...
ORGANISM(S): Saccharomyces cerevisiae 
2021-03-31 | E-MTAB-9521 | biostudies-arrayexpress
GenomicsGenome-wide maps of Nup98 binding in human embryonic stem cells, neural progenitors, neuron cells and lung fibroblasts
Recent evidence suggests that nucleoporins, well known components that control nucleo-cytoplasmic trafficking, have wide-ranging functions in developmental gene regulation that potentially extend beyond their role in nuclear transport. Whether the unexpected role of nuclear pore proteins in transcri...
ORGANISM(S): Homo sapiens 
2013-07-17 | E-GEOD-48996 | biostudies-arrayexpress
TranscriptomicsEffect of NPAP1/C15orf2 overexpression on transcriptome of HEK293 cells
To gain insight into the function of Nuclear pore associated protein 1 (NPAP1, formerly C15orf2), we overexpressed NPAP1 in HEK293 cells. We detected no significant difference between NPAP1-expression of induced and uninduced cells in three technical replicates, exept for an approximately 10-fold in...
ORGANISM(S): Homo sapiens 
2012-06-17 | E-GEOD-36575 | biostudies-arrayexpress
ProteomicsProximity ligation mapping of microcephaly associated SMPD4 shows association with components of the nuclear pore mem-brane
SMPD4 is a neutral sphingomylinase implicated in a specific type of congenital microcephaly. Although not intensively studied, SMPD4 deficiency has also been found to cause cell division defects. This suggests a role for SMPD4 in cell cycle and differentiation. In order to explore this role we used ...
ORGANISM(S): Homo sapiens (Human) 
2022-10-25 | PXD037694 | Pride
Nuclear pore membrane  sphingomyelinase  nup35  smpd4  n-smnase-3 
TranscriptomicsChromatin immunoprecipitation to identify gene-nuclear pore association in wild type and THO mutants of yeast
This work aimed at analysing the genomic loci that are aberrantly docked to the nucleopore in mutants of the THO complex
ORGANISM(S): Saccharomyces cerevisiae 
2008-08-15 | E-TABM-535 | biostudies-arrayexpress
ProteomicsMembrane protein landscape of plant nuclear envelope and nuclear pore complex
We used biochemical fractionation coupled with the subtractive proteomics to profile PNET proteins. Both the crude NE and the total MM fraction were subject to label-free quantitative mass spectrometry (LFQMS). We also adapted proximity labeling-coupled LFQMS (PL-LFQMS) method to map the PNET proteo...
ORGANISM(S): Arabidopsis Thaliana 
2020-03-11 | PXD015919 |
ProteomicsPNET1 is an on-demand component of the nuclear pore complex gating cell proliferation rates
The nuclear pore complex (NPC) is a cornerstone of eukaryotic cell functionality, orchestrating the nucleocytoplasmic shuttling of macromolecules. Here, we report that PNET1, a transmembrane nucleoporin conserved between humans and plants, is a dynamic, adaptable NPC component, exclusively expressed...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-03-10 | PXD054475 | Pride
Arabidopsis thaliana Callus Nuclear pore complex 
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