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GenomicsPartitioning the C. elegans genome by nucleosome modification, occupancy, and positioning
We have characterized two post-translational histone modifications in C. elegans on a genomic scale. Micrococcal nuclease digestion and immunoprecipitation were used to obtain distinct populations of single nucleosome cores, which were analyzed using massively parallel DNA sequencing to obtain pos...
ORGANISM(S): Caenorhabditis elegans 
2009-08-31 | E-GEOD-17284 | biostudies-arrayexpress
GenomicsGenome-wide nucleosome positioning during embryonic stem cell development [MNase-Seq]
We determined genome-wide nucleosome occupancy in mouse embryonic stem cells and their neural progenitor and embryonic fibroblast counterparts to assess features associated with nucleosome positioning during lineage commitment. Cell type and protein specific binding preferences of transcription fact...
ORGANISM(S): Mus musculus 
2012-10-21 | E-GEOD-40910 | biostudies-arrayexpress
TranscriptomicsDeterminants of nucleosome positioning and their influence on plant gene expression
We report the global nuclesome posiitons to study the relationship between nucleosome occupancy and gene expression in response to Coronatine in Arabidopsis We examined nucleosome occupancy and mRNA abundance in response to Coronatine in Arabidopsis rosette leaves
ORGANISM(S): Arabidopsis thaliana 
2015-05-27 | E-GEOD-64397 | biostudies-arrayexpress
GenomicsEffects of DNA Methylation on Nucleosome Stability
Methylation of DNA at CpG dinucleotides represents one of the most important epigenetic mechanisms involved in the control of gene expression in vertebrate cells. In this report, we conducted high-throughput nucleosome reconstitution experiments on 572 KB of human DNA and 668 KB of mouse DNA that wa...
ORGANISM(S): Mus musculus 
2013-01-28 | E-GEOD-37224 | biostudies-arrayexpress
GenomicsPING 2.0: An R/Bioconductor package for nucleosome positioning using next-generation sequencing data
MNase-Seq and ChIP-Seq have evolved as popular techniques to study chromatin and histone modification. Although many tools have been developed to identify enriched regions, software tools for nucleosome positioning are still limited. We introduce a flexible and powerful open-source R package, PING 2...
ORGANISM(S): Saccharomyces cerevisiae 
2013-07-11 | E-GEOD-47073 | biostudies-arrayexpress
GenomicsEffects of Histone H3 depletion on nucleosome occupancy and positioning through the S. cerevisiae genome [single-end MNase-seq]
Experiments performed over the past three decades have shown that nucleosomes are transcriptional repressors. In Saccharomyces cerevisiae, depletion of histone H4 results in the genome-wide transcriptional de-repression of hundreds genes. The mechanism of de-repression is hypothesized to be rooted d...
ORGANISM(S): Saccharomyces cerevisiae 
2012-09-26 | E-GEOD-29291 | biostudies-arrayexpress
TranscriptomicsRelationship between nucleosome positioning and DNA methylation: ChIP-chip
Nucleosomes compact and regulate access to DNA in the nucleus, and are composed of approximately 147 bases of DNA wrapped around a histone octamer1, 2. Here we report a genome-wide nucleosome positioning analysis of Arabidopsis thaliana utilizing massively parallel sequencing of mononucleosomes. By...
ORGANISM(S): Arabidopsis thaliana 
2010-05-28 | E-GEOD-21818 | biostudies-arrayexpress
GenomicsNucleosome positioning for 4 yeast species
We describe the genome-wide nucleosome profiles of four related yeast species. All species display the same global organization features first described in S. cerevisiae: a stereotypical nucleosome organization along genes, and the classification of promoters into these which contain or lack a prono...
ORGANISM(S): Saccharomyces cerevisiae 
2011-12-09 | E-GEOD-23577 | biostudies-arrayexpress
GenomicsComparison of nucleosome positioning among two yeast species and their hybrid for wild-type and deletion mutant strains
To study the evolution of nucleosome positioning we mapped nucleosome positioning in two species of yeasts. Identified differences in nucleosome positioning were classified into cis-based changes or trans-bseed changes based on the pattern of nucleosomes in the hybrid. This analysis was performed fo...
ORGANISM(S): Saccharomyces paradoxus 
2010-03-01 | E-GEOD-18939 | biostudies-arrayexpress
TranscriptomicsNucleosome Mapping in S. pombe Centromeres
A key element for defining the centromere identity is the incorporation of a specific histone H3, CENP-A, known as Cnp1p in S. pombe. Previous studies have suggested that functional S. pombe centromeres lack nucleosome arrays and may involve chromatin remodeling as a key step of kinetochore assembl...
ORGANISM(S): Schizosaccharomyces pombe 
2008-05-30 | E-GEOD-10742 | biostudies-arrayexpress
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