Label-free whole-cell proteomics. Quadruplicates of fibroblast cell lines of healthy control and COA5 mutation carrying patient to investigate complex IV assembly.
Mitochondrial ribosomes (mitoribosomes) have undergone substantial structural remodelling throughout evolution leading to distinct structural properties compared to their prokaryotic ancestors. Compared to their prokaryotic counterparts, mitoribosomes show a substantial loss of ribosomal RNA (rRNA),...