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GenomicsRNA-guided Location and Function of the Cas9 CRISPR Endonuclease in Mammalian Cells
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated enzyme Cas9 is an RNA-guided nuclease that has been widely adapted for genome editing in eukaryotic cells. However, the in vivo target specificity of Cas9 is poorly understood and most studies rely on in silico predicti...
ORGANISM(S): Mus musculus 
2014-08-29 | E-GEOD-57650 | biostudies-arrayexpress
GenomicsCovalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair
Based on the hypothesis that, enhancing the local concentration of donor oligos could increase the correction rates, we generated and tested novel CRISPR-Cas9 systems, in which the DNA repair template is covalently conjugated to Cas9 (RNPD system). To validate our results from the HEK293T reporter c...
ORGANISM(S): Mus musculus 
2018-06-04 | E-MTAB-6808 | biostudies-arrayexpress
TranscriptomicsIn vivo transcriptional activation using CRISPR-Cas9 in Drosophila
Identifying putative transcription factor target genes by combining CRISPR/Cas9-based transcriptional activation with RNAseq in Drosophila S2R+ cells. This study focuses on the transcription factors Twist and Snail, singly and together. RNA from Drosophila cells following CRISPR/Cas9-based activatio...
ORGANISM(S): Drosophila melanogaster 
2015-08-10 | E-GEOD-71430 | biostudies-arrayexpress
ProteomicsBacterial Protoplast-Derived Nanovesicles Carrying CRISPR Cas9 Tools Efficiently Re-educate Tumor-Associated Macrophages for Cancer Immunotherapy
we developed an in vivo CRISPR-Cas9 genome editing system that targets tumor-associated macrophages (TAMs) via bacterial protoplast-derived nanovesicles (NVs) decorated with a pH-responsive PEG conjugated polymer and galactosamine-conjugated ligand
ORGANISM(S): Escherichia Coli Bl21(de3) 
PXD045507 |
TranscriptomicsGenome-wide CRISPR-Cas9 screens reveal loss of redundancy between PKMYT1 and WEE1 in Glioblastoma stem-like cells
To identify new therapeutic targets for Glioblastoma (GBM), we performed genome-wide CRISPR-Cas9 "knockout" (KO) screens in patient-derived GBM stem-like cells (GSCs) and human neural stem/progenitors (NSCs), non-neoplastic stem cell controls, for genes required for their in vitro growth. ...
ORGANISM(S): Homo sapiens 
2016-02-15 | E-GEOD-70038 | biostudies-arrayexpress
ProteomicsCRISPR/Cas9-mediated knockout and endogenous complex analysis of Cluap1/ IFT38
CRISPR/Cas9-mediated gene-editing allows manipulation of a gene of interest in its own chromosomal context. When applied to the analysis of protein interaction, and in contrast to an exogenous expression of a protein, these can be studied maintaining physiological stochiometries, topology and contex...
ORGANISM(S): Homo sapiens (Human) 
2018-04-04 | PXD008343 | Pride
Cilia Proteomics Crispr/cas9 Ift 
ProteomicsUsing a Dual CRISPR/Cas9 Approach to Gain Insight into the Role of LRP1B in Glioblastoma
LRP1B remains one of the most altered genes in cancer, although its relevance in cancer biology is still unclear. Recent advances in gene editing techniques, particularly CRISPR/Cas9 systems, offer new opportunities to evaluate the function of large genes, like LRP1B. Using a dual sgRNA CRISPR/Cas9 ...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD042667 | Pride
Glioblastoma Ploidy Crispr/cas9 Lrp1b Secretome 
TranscriptomicsGenome-Wide Specificity of DNA-Binding, Gene Regulation, and Chromatin Remodeling by TALE- and CRISPR/Cas9-Based Transcription Factors
Synthetic DNA-binding proteins have found broad application in gene therapies and as tools for interrogating biology. Engineered proteins based on the CRISPR/Cas9 and TALE systems have been used to alter genomic DNA sequences, control transcription of endogenous genes, and modify epigenetic states. ...
ORGANISM(S): Homo sapiens 
2015-05-27 | E-GEOD-68341 | biostudies-arrayexpress
ProteomicsDeciphering the role of MFGE8 in lactation biology using CRISPR-CAS9 based gene editing in Buffalo mammary epithelial cells
Milk fat globule EGF factor 8 (MFGE8) is a glycoprotein which plays a crucial role in mammary gland remodeling. Our group previously identified MFGE8 as a marker associated with high milk yielding cows. Here, we generated MFGE8 knock-out buffalo mammary epithelial cells (BuMEC) via CRISPR-cas9 techn...
ORGANISM(S): Bos taurus (Bovine) 
2025-08-25 | PXD048149 | Pride
Crispr-cas9 Lactation biology Mfge8 
GenomicsCRISPR-Cas9 screening of lncRNAs and protein coding genes involved in the transdifferentiation from human pre-B cells into macrophages
BLaER1 cells are human leukemia pre-B cells able to transdifferentiate into functional and non-tumorigenic macrophages. With the goal of uncovering the genes involved in the transdifferentiation process, we have designed a DECKO (Double Excision CRISPR Knockout) library to knockout lncRNAs and prote...
ORGANISM(S): Homo sapiens 
2022-04-25 | E-MTAB-10445 | biostudies-arrayexpress
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