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ProteomicsAerobic Adaptation and Metabolic Dynamics of Propionibacterium freudenreichii DSM 20271: Insights from Comparative Transcriptomics and Surfaceome Analysis
Propionibacterium freudenreichii (PFR) DSM 20271 is a bacterium known for its ability to thrive in diverse environments and produce vitamin B12. Despite its anaerobic preference, recent studies have elucidated its ability to prosper in the presence of oxygen, prompting a deeper exploration of its ph...
ORGANISM(S): Propionibacterium freudenreichii subsp. freudenreichii 
2024-09-09 | PXD051703 | Pride
Vitamin b12 Aerobic adaptation Surfaceome analysis Propionibacterium freudenreichii 
ProteomicsPhosphoproteomic characterization of infulenza A virus infected human macrophages
Human primary macrophages were infected with influenza A virus (H3N2/Udorn strain, HA 256) for 6 hrs or left untreated. Cells were collected and lysed with HEPES lysis buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA, 1 % NP-40, pH 7.4) including protease and phosphatase inhibitor cocktails. The cell lys...
ORGANISM(S): Homo sapiens (Human) 
2016-08-08 | PXD001620 | Pride
Primary human macrophages Phosphorylation Influenza a virus 
ProteomicsProteomic and bioinformatic characterization of extracellular vesicles released from human primary macrophages upon influenza A infection
We have previously shown that IAV infection of human monocyte-derived macrophages causes major alterations to subcellular protein trafficking inside the cell and triggers robust protein secretion (Lietzén et al, 2011). Here, we have used high-throughput proteomics to identify proteins secreted in ex...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-16 | MSV000080643 | MassIVE
ProteomicsProteomic and bioinformatic characterization of extracellular vesicles released from human primary macrophages upon influenza A infection
We have previously shown that IAV infection of human monocyte-derived macrophages causes major alterations to subcellular protein trafficking inside the cell and triggers robust protein secretion (Lietzén et al, 2011). Here, we have used high-throughput proteomics to identify proteins secreted in ex...
ORGANISM(S): Homo sapiens (Human) 
2017-02-07 | PXD004424 | Pride
Lc-msms Extracellular vesicle Human primary macrophages Influenza a 
ProteomicsPhosphoproteomic characterization of infulenza A virus infected human macrophages
Human primary macrophages were infected with influenza A virus (H3N2/Udorn strain, HA 256) for 6 hrs or left untreated. Cells were collected and lysed with HEPES lysis buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA, 1 % NP-40, pH 7.4) including protease and phosphatase inhibitor cocktails. The cell lys...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080717 | MassIVE
ProteomicsProteomic and bioinformatic characterization of proteins secreted conventionally and unconventionally from human primary macrophages upon LPS transfection
The non-canonical caspase-4/5 inflammasome has been only very recently characterized. It is expressed by macrophages, the principal effector cells of the innate immunity system, and is activated by gram-negative bacteria, ER stress, and UV radiation. Activation of this inflammasome results in pyropt...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080769 | MassIVE
ProteomicsPhosFox: a software tool for peptide-level processing of LC-MS/MS-based phosphoproteomic data
Human keratinocytes, HaCaT cells were transfected with the dsRNA-analogue polyinosinic-polycytidylic acid (poly I:C) (Sigma-Aldrich) using Lipofectamine 2000 reagent (Invitrogen) for intracellular delivery. The cells were transfected with 7 ug/ml poly I:C for 1 h or left untreated. The cells were co...
ORGANISM(S): Homo sapiens (Human) 
2014-06-30 | PXD000577 | Pride
Peptide Phosphorylation Human Lc-ms/ms 
ProteomicsA MITOCHONDRIAL STRESS RESPONSE TRIGGERED BY DEFECTS IN PROTEIN SYNTHESIS QUALITY CONTROL
Quality control defects of mitochondrial nascent chain synthesis trigger a sequential stress response characterised by OMA1 activation and ribosome decay, determining mitochondrial form and function.
ORGANISM(S): Mus musculus (Mouse) 
2019-01-23 | PXD012416 | Pride
Mitochondrial disease  translation  ribosome  membrane  quality control 
ProteomicsDesign of a proteolytically stable sodium-calcium exchanger 1 (NCX1)-phospholemman (PLM) disruptor peptide for in vivo studies
The cardiac sodium–calcium exchanger (NCX1) is important for normal Na+ and Ca2+ homeostasis and cardiomyocyte relaxation and contraction. NCX1 activity is reduced by its endogenous inhibitor phosphorylated phospholemman (pSer68-PLM). Disrupting the pSer68-PLM-NCX1 interaction is therefore a potenti...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD022797 | Pride
Phospholemman  sodium-calcium exchanger  peptidomimetics  peptide array  cardiac 
ProteomicsSpry1 as a potential new hypoxia modulator in cutaneous melanoma
Introduction. At present, the precise role of Spry proteins in tumor progression is still debated. Our previous study showed that Spry1 knockdown (Spry1KO) in BRAFV600-mutant cutaneous melanoma (CM) promoted cell cycle arrest and apoptosis, repressed cell proliferation in vitro, and reduced tumor gr...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD038916 | Pride
Cutaneous melanoma Hypoxia Braf Ip-ms Spry1 
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