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ProteomicsThe OPTN interactome using BioID
We use a proximity labelling-based proteomics strategy (BioID) to map the interactome of OPTN. OPTN was tagged N and C-terminally with BirA* in RPE1 cells and then, after labelling with biotin, streptavidin pull downs were performed to identify OPTN-proximal proteins.
ORGANISM(S): Homo sapiens (Human) 
2020-06-16 | PXD019386 | Pride
Bioid Optn 
ProteomicsThe hTERT-RPE1 secretome after TLR3 stimulation
RPE cells perform a number of support functions in the inner eye including the secretion of signalling molecules and the maintenance of the immune privileged environment through communication with the immune system (Detrick and Hooks, 2010). Previous reports have demonstrated that RPE cells express ...
ORGANISM(S): Homo sapiens (Human) 
2020-06-16 | PXD019445 | Pride
Poly(i:c) Htert-rpe1 Human Secretome 
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