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TranscriptomicsDNA methylation comparison of ntTS, TS and ES cells
Placental abnormalities occur frequently in cloned animals. To investigate DNA methylation profile of trophoblast cell lineage of somatic cell nuclear transferred (NT) embryos, we established TS cells from blastocysts produced by NT at the blastocyst stage. Three independent TS cell lines derived fr...
ORGANISM(S): Mus musculus 
2010-05-16 | E-GEOD-17104 | biostudies-arrayexpress
TranscriptomicsGenome-wide transcriptome analyses by the RNA-seq method
We performed the whole transcriptome analysis in Zscan4 positive ES cells (Em+) and Zscan4 negative ES cells (Em-) by using FACS-sorted MC1-ZE7 ES cells. Whole RNA-seq in Zscan4 positive and negative cells
ORGANISM(S): Mus musculus 
2015-09-10 | E-GEOD-58619 | biostudies-arrayexpress
GenomicsChIP-seq assays for H3K9me2
We analyzed the genome-wide chromatin states in Zscan4 positive ES cells (Em+) and Zscan4 negative ES cells (Em-) by using FACS-sorted MC1-ZE7 ES cells. H3K27 hyperacetylation and DNA demethylation were detected in heterochromatic regions of Em+ cells. These results suggested that the heterochromati...
ORGANISM(S): Mus musculus 
2015-09-10 | E-GEOD-58617 | biostudies-arrayexpress
TranscriptomicsIsolated cardiomyocyte and cardiac fibroblast expression analysis
Some cell type-specific gene expression is maintained in the maturation of cardiomyocytes, where DNA hypomethylation of gene body regions of a set of specific genes. We used microarrays to detail the global gene expression program underlying the maintenance of cardiomyocyte function and maturation a...
ORGANISM(S): Mus musculus 
2012-07-05 | E-GEOD-39039 | biostudies-arrayexpress
GenomicsGenome-wide DNA methylation analyses by the HELP assay
The HELP tagging assay was performed on purified genomic DNAs. The protocol was modified for NEBNext Multiplex Oligos for Illumina (NEB) from the original protocol4 (http://wasp.einstein.yu.edu/index.php/Protocol:HELP_tagging). Briefly, genomic DNA was digested by HpaII or MspI, the former only cutt...
ORGANISM(S): Mus musculus 
2015-09-10 | E-GEOD-51680 | biostudies-arrayexpress
UnknownSize-exclusion chromatography-small-angle neutron scattering system optimized for an instrument with medium neutron flux.
Not available
S-EPMC11957415 | biostudies-literature
GenomicsDNA methylation profiles of isolated embryonic and adult cardiomyocytes, cardiac fibroblasts, tissue and stem cells.
To investigate the DNA methylation profiles of matured differentiated cardiomyocytes, DNA methylation profiles were obtained by HELP tagging assay using the isolated cardiomyocytes and cardiac fibroblasts from different developmental stages, tissues and cultured stem cells. DNA methylation in mouse ...
ORGANISM(S): Mus musculus 
2014-09-10 | E-GEOD-61249 | biostudies-arrayexpress
UnknownAccuracy for Mortality Prediction With Additive Biomarkers Including Interleukin-6 in Critically Ill Patients: A Multicenter Prospective Observational Study.
Not available
S-EPMC8078448 | biostudies-literature
UnknownRisk prediction of biomarkers for early multiple organ dysfunction in critically ill patients.
Not available
S-EPMC8573766 | biostudies-literature
UnknownA randomized, controlled, multicenter trial of the effects of antithrombin on disseminated intravascular coagulation in patients with sepsis.
Not available
S-EPMC4057033 | biostudies-literature
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