This model is from the article:
Temporal Coding of Insulin Action through Multiplexing of the AKT Pathway.
Kubota H, Noguchi R, Toyoshima Y, Ozaki Y, Uda S, Watanabe K, Ogawa W, Kuroda S. Mol Cell.
2012 Jun 29;46(6):820-32. 22633957
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In Saccharomyces cerevisiae, the Ca2+/calmodulin-dependent protein phosphatase, calcineurin, is activated by specific environmental conditions, including exposure to Ca2+ and Na+, and induces gene expression by regulating the Crz1p/Tcn1p transcription factor. We used DNA microarrays to perform a com...
In Saccharomyces cerevisiae, the Ca2+/calmodulin-dependent protein phosphatase, calcineurin, is activated by specific environmental conditions, including exposure to Ca2+ and Na+, and induces gene expression by regulating the Crz1p/Tcn1p transcription factor. We used DNA microarrays to perform a com...
Cells were cultured with or without FK506 treatment and exposed to 0.8 M NaCl. Samples were collected from the drug-treated and control cultures at 30 and 45 min after Na+ addition. For the publication, the experiment was repeated twice, and the corresponding time points from each experiment were ...
Cells were grown in YPD medium (pH 5.0) at 30oC to a density of 0.6 X 107 cells/ml (A600=0.6). The culture was supplemented with ET at 30 oC for 15 min. Cells were collected for the t=0 min sample. Next, an equal volume of YPD medium (pH 5.0) containing ET solution and 1.6 M NaCl was added to the ET...
Cells were grown in YPD medium (pH 5.0) at 30oC to a density of 0.6 X 107 cells/ml (A600=0.6). The culture was supplemented with FK506 (1.0 mg/ml) in ET at 30oC for 15 min. Cells were collected for the t=0 min sample. Next, an equal volume of YPD medium (pH 5.0) containing FK506 (1.0 mg/ml) and 1.6 ...
Wild-type strain DBY7286 and the crz1 mutant strain YHY804 were cultured and exposed to NaCl. Samples were collected at 30 and 45 min after Na+ addition. For the publication the experiment was repeated twice, and corresponding timepoints from each experiment were averaged. The first two arrays an...
Cells were grown at 30oC in YPD medium (buffered to pH 5.0 with 7.5 mM succinate) containing ET solution to a density of 0.6 X 107 cells/ml (A600 0.6). The culture was supplemented with FK506 (1.0 mg/ml final concentration) in ET at 30oC for 15 min. Cells were collected for the t=0 min sample. Next,...
Wild-type strain DBY7286 and the crz1D strain YHY804 were cultured and exposed to CaCl2 as described for Experiments 1 and 2. Samples were collected at 15 and 30 min after Ca2+ addition. For the publication, the experiment was repeated twice, and corresponding timepoints were averaged between the e...
Cells were grown at 30oC in YPD medium (buffered to pH 5.0 with 7.5 mM succinate) containing ET solution to a density of 0.6 X 107 cells/ml (A600 0.6). The culture was supplemented with ET at 30oC for 15 min. Cells were collected for the t=0 min sample. Next, an equal volume of YPD medium (pH 5.0) ...
