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Functionally distinct CD4+ helper T (Th) cell subsets, such as Th1, Th2, Th17, and regulatory T cells (Treg), play a pivotal role in the host-defense against pathogen invasion and the pathogenesis of inflammatory disorders. In this project, DIA-MS-based proteome analysis was performed on naïve CD4+ ...
ORGANISM(S): Mus Musculus (mouse) 
DLX3 is a homeodomain transcription factor involved in ameloblast differentiation and enamel formation. Mutations in DLX3 in human lead to defects in enamel. However, the downstream targets of Dlx3 transcriptional activity in the enamel organ have not been identified yet. In this study, we compared ...
ORGANISM(S): Mus musculus 
Genetic diagnosis plays a central role in the clinical management of patients with inborn errors of immunity (IEI). We proposed a novel method for diagnosing IEI using PBMC proteomics integrated with targeted RNA sequencing, providing notable insights into the pathogenesis of IEI. Data-independent a...
ORGANISM(S): Homo Sapiens (human) 
We developed a simple and sensitive analysis method for dried blood spot (DBS) proteins by sodium carbonate precipitation (SCP). We aimed to removing large amounts of hydrophilic blood proteins such as hemoglobin, albumin, fibrinogen and transferrin in DBS using a simple pretreatment with sodium car...
ORGANISM(S): Homo Sapiens (human) 
Caecal contents from three specific pathogen-free (SPF) mice, and three germ-free (GF) mice were analyzed by DIA-MS (SWATH-MS) using TripleTOF 5600+ (SCIEX). Paraprevotella clara (P. clara) culture supernatants nontreated and treated tunicamycin or 2-fluro-L-fucose (2F-Fuc) were analyzed by DIA-MS u...
ORGANISM(S): Paraprevotella Clara Mus Musculus (mouse) 
00502 protein derived from Paraprevotella clara, as well as mixture of 00502 protein and human PRSS2 were analyzed by Native-PAGE. The observed bands were cut out and digested in-gel. The digested peptides were analyzed by DDA-MS using Q Exactive HF-X .
ORGANISM(S): Paraprevotella Clara 
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