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Total RNA-seq data from samples produced using conventional TRIzol RNA extraction (control), using the semi-extractability assay (PMID: 28404604) or Orthogonal Organic Phase Separation (OOPS; PMID: 30607034, PMID: 32651564) in naive mESCs (nPSCs), primed pluripotent stem cells (pPSCs) and 1-day Wnt-...
ORGANISM(S): Mus musculus 
Total RNA-seq data from samples produced using conventional TRIzol RNA extraction (control), using the semi-extractability assay (PMID: 28404604) or Orthogonal Organic Phase Separation (OOPS; PMID: 30607034, PMID: 32651564) in naive mESCs (nPSCs). Control samples RNA was extracted from the aqueous p...
ORGANISM(S): Mus musculus 
Total RNA-seq data from samples produced using conventional TRIzol RNA extraction (control), using the semi-extractability assay (PMID: 28404604) or Orthogonal Organic Phase Separation (OOPS; PMID: 30607034, PMID: 32651564) in naive mESCs (nPSCs) after the cells were exposed to 10 μM thapsigargin fo...
ORGANISM(S): Mus musculus 
Comprehensive identification of RNA–protein interactions in any organism using orthogonal organic phase separation (OOPS)s
RNA-seq for identifying semi-extractable and OOPS RNAs in naive mouse embryonic stem cells (second batch)
Identification of RNA–protein interactions currently requires a quantity and quality of sample that precludes their widespread application, especially for dynamic biological systems or precious samples. Here, we present Orthogonal Organic Phase Separation (OOPS), a new approach to enrich RNA Binding...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-12-17 | PXD009668 | Pride
RNA-seq for identifying semi-extractable and OOPS RNAs in naive and primed mouse embryonic stem cells
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