The quadrupole Orbitrap mass spectrometer (Q Exactive) has made a powerful proteomics instrument available in a benchtop format. It significantly boosted analyzable proteins per hour and has now evolved into a proteomics analysis workhorse for many laboratories. Here we describe the Q Exactive Plus ...
UVPD was implemented on an Orbitrap Q-Exactive plus equipped with a ESI/EP-MALDI. UVPD of MALDI generated ions was benchmarked against MALDI-ISD, MALDI-HCD and ESI-UVPD. MALDI UVPD outperformed MALDI HCD and ISD efficiently sequencing small proteins ions.
ORGANISM(S): Homo sapiens (Human) Equus caballus (Horse)
The quadrupole Orbitrap mass spectrometer (Q Exactive) has made a powerful proteomics instrument available in a benchtop format. It significantly boosted analyzable proteins per hour and has now evolved into a proteomics analysis workhorse for many laboratories. Here we describe the Q Exactive Plus ...
The bloom-forming diatoms Coscinodiscus granii were cultivated in a non-contact dual cultivation apparatus. This device allows the exchange of exudates, without transfer of any microorganisms, allowing the investigation of both endo- and exo-metabolome. The treatment consisted in infecting diatom ce...
E-Jiao is a traditional Chinese medicine. In this project, we used Q Exactive Orbitrap Plus to identify the peptides from E-Jiao. We also used the QE Plus to investigate the E-Jiao peptides in rat blood.
We compared the performance of multidimensional protein identification (MudPIT) on Velos Pro Orbitrap (VPO) and Velos Orbitrap Elite (VOE) mass spectrometers to single dimension reversed phase (RP) chromatography on a Q Exactive Plus (QE+) and an Orbitrap Fusion™ Lumos™ (OFL). Using a digested HeLa...
To expand the knowledge on the porcine salivary proteome, secretions from the three major salivary glands were collected from anaesthetised piglets. Pilocarpine and isoproterenol were simultaneously injected intraperitoneally to increase the volume and protein concentration of the saliva, respective...
The faster sequencing speed available on the latest Q Exactive HF mass spectrometer is investigated and evaluated by four different acquisition methods and benchmarked across three generations of Q Exactive instruments. Also the instrument capabilities for offline high pH reversed-phase peptide frac...
