Sort   by:  
 Page size 
ProteomicsFunctional partitioning of a liquid-like organelle during assembly of axonemal dyneins
The associated files are the results of AP-MS experiments done from Xenopus multiciliated cells derived from dissected animal caps. Baits used for affinity purification were GFP-dnai2, GFP-wdr78, and GFP-C16orf71. Note that some files associated with the C16orf71 experiments may be mislabeled C16o...
ORGANISM(S): Xenopus Laevis (ncbitaxon:8355) 
2020-03-10 | MSV000085075 | MassIVE
TranscriptomicsA native chromatin purification system for epigenomic profiling in C. elegans
We describe an in vivo chromatin purification system for genome-wide epigenetic profiling in C. elegans. In this system, we coexpressed the E. coli biotin ligase enzyme (BirA), together with the C. elegans H3.3 gene fused to BioTag, a 23-amino acid peptide serving as a biotinylation substrate for Bi...
ORGANISM(S): Caenorhabditis elegans 
2010-05-19 | E-GEOD-18898 | biostudies-arrayexpress
ProteomicsLysosomal proteomics by affinity purification-mass spectrometry
The aims of this project is to establish a comprehensive proteomic map of the lysosomes using isolated lysosomes via affinity purification. Affinity-purified mitochondria was used as a control to demonstrate the organelle specificity. In this study, we identified several lipid transporters that are ...
ORGANISM(S): Homo sapiens (Human) 
2019-09-27 | PXD014733 | Pride
Mtorc1 Organelle Lysosome immunoprecipitation 
UnknownImproved expression and purification of sigma 1 receptor fused to maltose binding protein by alteration of linker sequence.
Not available
S-EPMC3679933 | biostudies-literature
ProteomicsR7 Soybean seed organelle enrichment proteomics
This data is part of a study examining the results of increased malic enzyme activity in soybean seeds. The study characterizes changes to oil content and free amino acids when malic enzyme is expressed in different subcellular locations. Malic enzyme was expressed using seed-specific promoters to t...
ORGANISM(S): Glycine max 
2023-03-22 | PXD039994 | Pride
R7 seed Glycine max Organelle enrichment 
TranscriptomicsRNA-seq analysis of differentiating human erythroblasts
Human erythroblasts purified from cord blood were cultured in vitro and FACS-sorted into five highly purified populations representing distinct differentiation stages:  proerythroblasts, early basophilic erythroblasts, late basophilic erythroblasts, polychromatophilic erythroblasts, and orthochromat...
ORGANISM(S): Homo sapiens 
2013-12-25 | E-GEOD-53635 | biostudies-arrayexpress
ProteomicsGlobal organelle profiling reveals subcellular localization and remodeling at proteome scale
Defining the subcellular distribution of all human proteins and its remodeling across cellular states remains a central goal in cell biology. Here, we present a high-resolution strategy to map subcellular organization using organelle immuno-capture coupled to mass spectrometry. We apply this proteom...
ORGANISM(S): Homo sapiens (Human) Human coronavirus OC43 
2023-12-19 | PXD046440 | Pride
Human Infection Oc43 Ip-ms 
ProteomicsIdentification of Silkworm Organelle Proteome
Isolate silkworm proteins in organelles of nucleus, cytosol, mitochondria and endoplasmic reticulum , and determine the proteins using mass spectrum
ORGANISM(S): Bombyx Mori 
2021-05-06 | PXD025816 |
ProteomicsA Proximity Labeling Strategy Enables Spatial Proteomic Analysis of Inter-organelle Membrane Contacts in Living Cells
Inter-organelle membrane contacts especially mitochondria-endoplasmic reticulum contacts (MERC) conduct important biological functions including exchange of lipids and ions, and modulation of membrane dynamics. Alterations of inter-organelle membrane contacts have been implicated with the pathogenes...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD035801 | Pride
Apex Bifc Merc Proteome 
UnknownApplication of proteomic marker ensembles to subcellular organelle identification.
Not available
S-EPMC2830848 | biostudies-literature
Sort   by:  
 Page size