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MetabolomicsCharacterization of Whole Blood EVs Using PRotein Organic Solvent PRecipitation

Blood plays a vital role in diagnostic medicine by providing essential information for assessing health and diagnosing various conditions. Routine blood tests can reveal important biomarkers that support early diagnosis and timely intervention. Recently, extracellular vesicles (EVs) have emerged ...

2026-08-24 | MTBLS12801 | MetaboLights
OtherExtracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR).
Data from ProteomeXchange, PXD ID: PXD002668. File: UltraCushion_R1_28.mzml. Published as part of Sci Rep. 2015 Sep 30;5:14664 . From the Abstract: {{i}} Extracellular vesicles (EVs) such as exosomes and microvesicles mediate intercellular communication and regulate a diverse range of crucial biolo...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32310024861 | GPMDB
Resource Reanalysis 
ProteomicsCovalent Organic Framework with Acid Precipitation Enhances Microprotein Enrichment and Discovery of Ferroptosis-Associated Microproteins
Microproteins, defined as polypeptides fewer than 100 amino acids, are encoded by small open reading frames (sORFs) and exhibit unique structural and functional characteristics. Despite their short length and recent discoveries, microproteins have already been found to play critical roles in diverse...
ORGANISM(S): Homo Sapiens 
2026-02-26 | PXD074935 |
ProteomicsSolvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads
Complete, reproducible extraction of protein material is essential for comprehensive and unbiased proteome analyses. A current gold standard is single-pot, solid-phase-enhanced sample preparation (SP3), in which organic solvent and magnetic beads are used to denature and capture proteins, with subse...
ORGANISM(S): Homo sapiens (Human) 
2022-08-04 | PXD028736 | Pride
Proteomics Solvent precipitation Sample preparation 
ProteomicsCharacterization of Whole Blood EVs Using PRotein Organic Solvent PRecipitation
Blood plays a vital role in diagnostic medicine by providing essential information for assessing health and diagnosing various conditions. Routine blood tests can reveal important biomarkers that support early diagnosis and timely intervention. Recently, extracellular vesicles (EVs) have emerged as ...
ORGANISM(S): Homo sapiens (Human) 
2026-08-25 | PXD066173 | Pride
Whole blood Human Extracellular vesicles Prospr 
ProteomicsOrganic molecules are involved in biomineralization by Streptomyces mirabilis
The Gram-positive, filamentous soil bacterium S. mirabilis P16B-1 has been shown to tolerate environmental concentrations of over 100 mM of Ni. The extremely high metal tolerance is partly provided through biomineralization observed for both Mg bearing struvite and Ni-struvite where Ni ions replace ...
ORGANISM(S): Streptomyces mirabilis 
2026-07-07 | PXD049698 | Pride
Struvite Biomimetic mineral formation Metabolome Biomineralization Nickel Streptomyces Proteome 
ProteomicsSolvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads
Complete, reproducible extraction of protein material is essential for comprehensive and unbiased proteome analyses. A current gold standard is single-pot, solid-phase-enhanced sample preparation (SP3), in which organic solvent and magnetic beads are used to denature and capture protein aggregates, ...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) Drosophila melanogaster (Fruit fly) 
2022-08-01 | PXD032095 | Pride
Microparticles Proteomics Sample preparation Protein aggregation Contaminant removal Lc−ms/ms Protein precipitation 
ProteomicsSolvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads
Complete, reproducible extraction of protein material is essential for comprehensive and unbiased proteome analyses. A current gold standard is single-pot, solid-phase-enhanced sample preparation (SP3), in which organic solvent and magnetic beads are used to denature and capture proteins, with subse...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-03 | PXD028768 | Pride
Microparticles Protein aggregation Contaminant removal Lc−ms/ms Protein precipitation Sample preparation 
ProteomicsIsolation of extracellular vesicles by PROSPR
Here, we report a novel and inexpensive method of rapidly isolating EVs from small volumes of human blood plasma by PRotein Organic Solvent PRecipitation (PROSPR). PROSPR encompasses a rapid three-step protocol to remove soluble proteins from plasma via precipitation in cold acetone, leaving the lip...
ORGANISM(S): Homo sapiens (Human) 
2015-10-05 | PXD002668 | Pride
Blood plasma Extracellular vesicles Exosome Prospr Biochemical purification Biomarker 
UnknownFrom Adsorption to Precipitation of U(VI): What is the Role of pH and Natural Organic Matter?
Not available
S-EPMC8680647 | biostudies-literature
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