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MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
MetabolomicsOnline Headspace-Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry-based untargeted volatile metabolomics for studying emerging complex biopesticides: A proof of concept
This work introduces a novel online Headspace-Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry-based untargeted metabolomics approach, suggested as an alternative tool to study the environmental fate of volatile xenometabolites in emerging complex biopesticides, e.g. the Myrica gale ...
2021-01-26 | MTBLS2026 | MetaboLights
ProteomicsOrthogonal Organic Phase Separation protocol (2018)
Identification of RNA–protein interactions currently requires a quantity and quality of sample that precludes their widespread application, especially for dynamic biological systems or precious samples. Here, we present Orthogonal Organic Phase Separation (OOPS), a new approach to enrich RNA Binding...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-12-17 | PXD009668 | Pride
Rna-binding Human Oops Rbpome Proteome E.coli 
MetabolomicsSponges With Microbial Symbionts Transform Dissolved Organic Matter and Take Up Organohalides (2019 collection assay)

Seawater dissolved organic matter (DOM) is a large reservoir of carbon composed of a complex and poorly characterized mixture of molecules. Sponges have long been known to consume dissolved organic carbon (DOC) from this mixture, but the role of microbial sponge symbionts in this process is compl...

2022-03-11 | MTBLS2200 | MetaboLights
MetabolomicsSponges With Microbial Symbionts Transform Dissolved Organic Matter and Take Up Organohalides (2018 collection assay)

Seawater dissolved organic matter (DOM) is a large reservoir of carbon composed of a complex and poorly characterized mixture of molecules. Sponges have long been known to consume dissolved organic carbon (DOC) from this mixture, but the role of microbial sponge symbionts in this process is compl...

2022-03-11 | MTBLS2199 | MetaboLights
GenomicsComprehensive identification of RNA–protein interactions in any organism using orthogonal organic phase separation (OOPS)
Comprehensive identification of RNA–protein interactions in any organism using orthogonal organic phase separation (OOPS)s
PRJEB26736 | ENA
GenomicsTotal RNA-seq of L. mexicana promastigotes and axenic amastigotes following UV-cross-linking and orthogonal organic phase separation
Total RNA-seq of L. mexicana promastigotes and axenic amastigotes following UV-cross-linking and orthogonal organic phase separation
PRJNA778524 | ENA
TranscriptomicsTotal RNA-seq of L. mexicana promastigotes and axenic amastigotes following UV-cross-linking and orthogonal organic phase separation
We compared the relative abundance of RNAs in the UV- cross-linked (CL) and non-cross-linked (NC) samples using total RNA-seq of both promastigotes and axenic amastigotes of L. mexicana parasites. Irradiation of the Leishmania parasites with UV-dose of 525 mJ/cm2 followed by sequential AGPC phase pa...
ORGANISM(S): Leishmania mexicana 
2022-01-14 | GSE188357 | GEO
MetabolomicsMetabolomic profiling of Campylobacter jejuni with resistance gene ermB by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry and tandem quadrupole mass spectrometry
The metabolome changes of Campylobacter jejuni with resistant gene ermB remain unclear. Here, we described an untargeted metabolomic workflow based on ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry to investigate the metabolites perturbations mediated by erm...
2018-08-07 | MTBLS496 | MetaboLights
ProteomicsRemodeling of RNA-Binding Proteome and RNA-mediated regulation as a new layer of control of sporulation
Sporulation allows certain bacteria to survive extreme conditions for extended periods posing challenges to public health and food safety. Transcriptional level of regulation relying on σ factors has been well studied in the non-pathogenic model Bacillus subtilis, while post-transcriptional control...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 Bacteria 
2025-08-13 | PXD061929 | Pride
Rna binding proteome Orthogonal organic phase separation Ooops Rbpome Bacillus subtilis Sporulation 
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