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TranscriptomicsTranscription profiling of S. cerevisiae paf1 and ctr9 mutants to identify direct targets of the Paf1/RNA polymerase II complex
To identify the direct targets of the Paf1/RNA polymerase II complex we compared expression profiles of isogenic wild type and paf1 and ctr9 mutant strains. We also created a Tet-regulated form of Paf1 and monitored expression patterns after shut off of Paf1. Samples were isolated at one hour interv...
ORGANISM(S): Saccharomyces cerevisiae 
2007-09-19 | E-GEOD-3200 | biostudies-arrayexpress
TranscriptomicsTEF-seq/NET-seq applied to factors Rpb3, Paf1, Spt6, Spt16, Cet1, Pcf11, Ssu72, Set2
Here we adapt native elongating transcript sequencing (NET-seq) to develop transcription elongation factor associated nascent elongating transcript sequencing (TEF-seq). In this the RNA polymerase II (Pol2) transcription elongation complex (TEC) is immunoprecipitated via associated transcription elo...
ORGANISM(S): Saccharomyces cerevisiae 
2021-01-18 | E-MTAB-4568 | biostudies-arrayexpress
TranscriptomicsEffects of innate immune stimuli on PAF1 knockout cells
PAF1 knockout and ncgRNA control cells were treated with multiple innate immune stimuli for 3 hours to mimic various microbial stresses.
ORGANISM(S): Homo sapiens 
2022-04-10 | E-MTAB-11620 | biostudies-arrayexpress
TranscriptomicsEffects of the Paf1 complex and histone modifications on snoRNA 3'-end formation reveal broad and locus-specific regulation.
High-density tiling arrays were used to compare transcriptional profiles between the paf1 deletion strain and a wild-type reference strain. This included assessment of signal within ORFs, as well as read-through transcription associated with each of the snoRNAs annotated in SGD. For the latter, hybr...
ORGANISM(S): Saccharomyces cerevisiae 
2012-10-26 | E-MTAB-1342 | biostudies-arrayexpress
GenomicsPAF1, a molecular regulator of promoter-proximal pausing by RNA Polymerase II
The control of promoter-proximal pausing and the release of RNA polymerase II (RNA Pol II) is a widely used mechanism for regulating gene expression in metazoans, especially for genes that respond to environmental and developmental cues. Here, we identify Pol II associated Factor 1 (PAF1) as a major...
ORGANISM(S): Drosophila melanogaster 
2015-08-13 | E-GEOD-70408 | biostudies-arrayexpress
ProteomicsElevated PAF1-RAD52 Axis Confers Chemoresistance to Human Cancers
To identify a common protein that interacts with PAF1 in ovarian cancer, lung Cancer, and pancreatic cancer to mediate chemoresistance.
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD039281 | Pride
Ovarian cancer Paf1 Pancreatic cancer Lung cancer Rad52 
TranscriptomicsGenome-wide expression profiling in 293T cells upon depletion of H1.2, Cul4A or PAF1
Increasing evidence suggests that linker histone H1 can influence distinct cellular processes by acting as a gene-specific regulator. However, the mechanistic basis underlying such H1 specificity and whether H1 acts in concert with other chromatin-altering activities remain unclear. To investigate t...
ORGANISM(S): Homo sapiens 
2013-12-20 | E-GEOD-52544 | biostudies-arrayexpress
ProteomicsThe PAF1 complex promotes 3‘ processing of pervasive transcripts
The PAF1 complex (PAF1C) functions in multiple transcriptional processes involving RNA Polymerase II (Pol II). eRNAs and PROMPTs are pervasive transcripts transcribed by Pol II and rapidly degraded by the nuclear exosome complex after 3’ endonucleolytic cleavage by the Integrator complex (Integrator...
ORGANISM(S): Homo sapiens (Human) 
2022-03-03 | PXD031531 | Pride
Transcription regulation  enhancer rna  3' end processing  paf1 complex  integrator complex  pervasive transcription 
Proteomics3x-HA Tagged PAF1 IP-Ms in MOLM-13 CAS9 cells
Transcriptional regulatory proteins are frequent drivers of oncogenesis and common targets for drug discovery. The transcriptional co-activator, ENL, which binds to chromatin through an acetyllysine reader YEATS domain, is preferentially required for the survival and pathogenesis of acute leukemia. ...
ORGANISM(S): Homo sapiens (Human) 
2025-10-21 | PXD066867 | Pride
Human Acute meyloid leukemia Transcriptional regulation 
UnknownPaf1 and Ctr9 subcomplex formation is essential for Paf1 complex assembly and functional regulation.
Not available
S-EPMC6143631 | biostudies-literature
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