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TranscriptomicsThe viral and cellular microRNA targetome in lymphoblastoid cell lines
Epstein-Barr virus (EBV) is a human herpesvirus linked to a number of B cell cancers and lymphoproliferative disorders. During latent infection, EBV expresses 25 viral pre-microRNAs (miRNAs) and induces the expression of specific host miRNAs, such as miR-155 and miR-21, which potentially play a role...
ORGANISM(S): Homo sapiens 
2012-10-16 | E-GEOD-41437 | biostudies-arrayexpress
ProteomicsMass spectrometry analysis of FLAG-5MP complexes
FLAG-tagged 5MP (h5MP1/BZW2 from human and 5MP/Kra from fly) was ectopically expressed in relevant host cells (HEK293T for human and S2 for fly) by transient transfection, and one-step affinity-purified by anti-FLAG affinity resin.The one-step affinity purification products were resolved by SDS-PAGE...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2021-05-27 | PXD026284 | JPOST Repository
TranscriptomicsTranscription profiling of E.coli BW25113 ymgB and W/T strains LBglu 24h biofilm cells
For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms (30) in 250 mL in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures diluted that were 1:167. The cells were shaken at 250 rpm and 37°C for 24 hours to form biofil...
ORGANISM(S): Escherichia coli 
2008-06-15 | E-GEOD-6925 | biostudies-arrayexpress
ProteomicsQuantitative chemical proteomic profiling of phosphoprotein phosphatases
We have developed a chemical proteomic strategy for the systematic interrogation of endogenous phosphoprotein phosphatases (PPP) and their interacting proteins, including regulatory and scaffolding subunits, and substrates (the 'PPPome'). PPPs are captured using an immobilized non-selective PPP inhi...
ORGANISM(S): Neurospora Crassa (ncbitaxon:5141) Candida Albicans (ncbitaxon:5476) Saccharomyces Cerevisiae (ncbitaxon:4932) Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) Schizosaccharomyces Pombe (ncbitaxon:4896) 
2017-07-07 | MSV000081226 | MassIVE
ProteomicsSmell of Infection: a novel, non-invasive method for detection of fish excretory- secretory proteins
Chemical signals are produced by aquatic organisms following predatory attacks or perturbations such as parasitic infection. Ectoparasites feeding on host fish are likely to cause release of similar alarm cues into the environment due to the stress, wounding and immune response stimulated upon infec...
ORGANISM(S): Kryptolebias marmoratus 
2019-01-02 | PXD010987 | Pride
Gel free ms Alarm cues Parasitic infection 
ProteomicsUnresolved stalled ribosome complexes restrict cycle progression after genotoxic stress
Translation of damaged mRNA can lead to ribosome stalling, thereby producing incomplete proteins toxic to the cell. The mechanism of ribosome-associated quality control (RQC) disassembles stalled ribosomes through the actions of the ASC-1 complex (ASCC). Here, we show that some reagents that chemica...
ORGANISM(S): Homo sapiens (Human) 
2022-02-07 | PXD023878 | Pride
Rna damage Rna interactome capture Ascc3 Asc-1 complex Ribosome-associated quality control 
ProteomicsUnresolved stalled ribosome complexes restrict cycle progression after genotoxic stress
Translation of damaged mRNA can lead to ribosome stalling, thereby producing incomplete proteins toxic to the cell. The mechanism of ribosome-associated quality control (RQC) disassembles stalled ribosomes through the actions of the ASC-1 complex (ASCC). Here, we show that some reagents that chemica...
ORGANISM(S): Homo sapiens (Human) 
2022-02-07 | PXD023976 | Pride
Rna damage Rna interactome capture Ascc3 Asc-1 complex Ribosome-associated quality control 
ProteomicsUnresolved stalled ribosome complexes restrict cycle progression after genotoxic stress
Translation of damaged mRNA can lead to ribosome stalling, thereby producing incomplete proteins toxic to the cell. The mechanism of ribosome-associated quality control (RQC) disassembles stalled ribosomes through the actions of the ASC-1 complex (ASCC). Here, we show that some reagents that chemica...
ORGANISM(S): Homo sapiens (Human) 
2022-02-07 | PXD023978 | Pride
Rna damage Rna interactome capture Ascc3 Asc-1 complex Ribosome-associated quality control 
ProteomicsUnresolved stalled ribosome complexes restrict cycle progression after genotoxic stress
Translation of damaged mRNA can lead to ribosome stalling, thereby producing incomplete proteins toxic to the cell. The mechanism of ribosome-associated quality control (RQC) disassembles stalled ribosomes through the actions of the ASC-1 complex (ASCC). Here, we show that some reagents that chemica...
ORGANISM(S): Homo sapiens (Human) 
2022-02-07 | PXD023861 | Pride
Rna damage Rna interactome capture Ascc3 Asc-1 complex Ribosome-associated quality control 
ProteomicsIdentifying secondary processes implicated in nemaline myopathy pathophysiology
Nemaline myopathy (NM) is a genetically and clinically heterogeneous disease that is diagnosed based on the presence of nemaline rods on skeletal muscle biopsy. While NM has typically been classified by causative genes, disease severity or prognosis cannot be predicted well. The common pathological ...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-06 | PXD042201 | Pride
Nemaline myopathy Neb cko Acta1 
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