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MetabolomicsGeneration and characterization of thiol-deficient Mycobacterium tuberculosis mutants
Mycothiol (MSH) and ergothioneine (ERG) are thiols able to compensate for each other to protect mycobacteria such as Mycobacterium smegmatis against oxidative stress. Gamma-glutamylcysteine (GGC), another thiol and an intermediate in ERG biosynthesis has detoxification abilities. Five enzymes are in...
2018-07-05 | MTBLS661 | MetaboLights
ProteomicsDevelopment of a capillary electrophoresis coupled to mass spectrometry pipeline for comparable assessment of the plasma peptidome
Although capillary electrophoresis coupled to mass spectrometry (CE-MS) holds promise for urinary peptide profiling, only a limited number of studies have used CE-MS to study plasma peptides. Here we describe the establishment of a workflow, including sample preparation, CE-MS and MS/MS analyses, da...
ORGANISM(S): Homo sapiens (Human) 
2025-05-16 | PXD056224 | Pride
Chronic kidney disease Plasma Cardiovascular diseases Peptidome Biomarkers Ce-ms/ms 
ProteomicsIdentification of HER2 peptides for quantification in formalin-fixed paraffin-embedded breast cancer tissues
The ability to accurately quantify proteins in formalin-fixed paraffin-embedded (FFPE) tissues using targeted mass spectrometry opens exciting perspectives for biomarker discovery. We have developed and evaluated a selected reaction monitoring (SRM) assay for the human receptor tyrosine-protein kina...
ORGANISM(S): Homo sapiens (Human) 
2015-07-16 | PXD002281 | Pride
Breast cancer Human receptor tyrosine-protein kinase erbb-2 (her2) Human Ltq orbitrap velos Formalin-fixed parrafin-embedded tissues (ffpe) 
ProteomicsThe Amino-Terminal Part of Human Filaggrin-2 is a Component of Cornified Envelopes
Filaggrin-2 (FLG2), a member of the S100-fused type protein family, is a 250 kDa protein mainly expressed in differentiated keratinocytes of the epidermis (Wu et al., 2009; Hsu et al., 2011). Its expression is reduced in atopic dermatitis (Pellerin et al., 2013), and heterozygous non-sense mutations...
ORGANISM(S): Homo sapiens (Human) 
2019-02-25 | PXD011833 | Pride
Keratinocyte Proteomics Corneocyte Transglutaminase Terminal differentiation Epidermis 
ProteomicsIGDQ motogenic peptide gradient induces directional cell migration through integrin (αv)β3 activation in metastatic breast cancer
In the context of breast cancer metastasis study, we have shown in an in vitro model of cell migration that IGDQ-exposing (IsoLeu-Gly-Asp-Glutamine type I Fibronectin motif) monolayers (SAMs) on gold sustain the adhesion of MDA-MB-231 cells by triggering Focal Adhesion Kinase by activating integrins...
ORGANISM(S): Homo sapiens (Human) 
2022-11-08 | PXD024540 | Pride
Breast cancer  tnbc  cell migration  motogenic igdq motif  fibronectin type i  integrin alpha 5  integrin beta 3 
ProteomicsHA-MOP knockin mice express the canonical µ-opioid receptor but lack detectable splice variants
G protein-coupled receptors (GPCRs) are notoriously difficult to detect in native tissues. In an effort to resolve this problem, we have developed a novel mouse model by fusing the hemagglutinin (HA)-epitope tag sequence to the amino-terminus of the µ-opioid receptor (MOP). Although HA-MOP knock-in ...
ORGANISM(S): Mus musculus (Mouse) 
2022-12-07 | PXD027825 | Pride
Brain Mouse Pharmacology Mop splice variants Opioid receptor family Nanolc-ms/ms cid/etd Phosphorylation 
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