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ProteomicsIn situ identification of S-sulfenylation sites in Arabidopsis thaliana using the genetic YAP1 probe
Hydrogen peroxide (H2O2) reacts, directly or indirectly, with cysteines to form cysteine sulfenic acid, also known as S-sulfenylation. This cysteine oxidation steers diverse cellular processes by altering protein interactions, trafficking, conformation, and function. We present a method to identify ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-12-15 | PXD016723 | Pride
S-sulfenylation Arabidopsis thaliana Post-translational modification Disulfide Cross-link Redox Cysteine oxidation Yap1c 
ProteomicsProtein S-sulfenylation Arabidopsis - AtMAPK4
Study the oxidation-sensitivity of recombinant AtMAPK4 by treatment with 0 mM hydrogen peroxide (H2O2) (sample '_01'), 1 mM (sample '_02'), and 10 mM (sample '_03'). Each sample subdivided for trypsin and chymotrypsin digestion.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-09-16 | PXD013588 | Pride
Arabidopsis thaliana Mitogen-activated protein kinase S-sulfenylation Atmapk4 Mpk4 Cysteine oxidation 
TranscriptomicsThe MOF-containing NSL complex associates globally with housekeeping genes, but activates only a defined subset (RNAi)
The MOF-containing NSL complex binds to many but not all promoters of active genes and potentially contributes to their proper gene expression. It is currently unknown what determines whether an active gene is bound or not. Here, we provide evidence that the NSL complex primarily targets active prom...
ORGANISM(S): Drosophila melanogaster 
2011-10-28 | E-GEOD-30990 | biostudies-arrayexpress
TranscriptomicsPSI-seq to Identify Sites of Pseudouridylation in S. cerevisiae
Using a technique based on the ability of CMC to specifically label pseudouridines and to stop reverse transcriptase, we provide a transcriptome-wide map of pseudouridylation in S. cerevisiae under log phase and heat shock conditions 4 conditions: log phase (2x), heat shock, and stationary phase. Fo...
ORGANISM(S): Saccharomyces cerevisiae 
2014-08-24 | E-GEOD-60445 | biostudies-arrayexpress
TranscriptomicsGene expression changes of Saccharomyces cerevisiae to linoleic acid hydroperoxide
Reactive oxygen species, generated in vivo or exogenously encountered, constantly challenge living organisms. Oxidation of polyunsaturated fatty acids (PUFA), which are susceptible to oxidant attack, can lead to initiation of lipid peroxidation and in turn rapid production of toxic lipid hydroperoxi...
ORGANISM(S): Saccharomyces cerevisiae 
2013-06-12 | E-GEOD-47820 | biostudies-arrayexpress
TranscriptomicsResponse of Saccharomyces cerevisiae (Dal80M-NM-^T) to linoleic acid hydroperoxide
Oxidative stress is experienced by all aerobic organisms and results in cellular damage. The damage caused during oxidative stress is particular to the oxidant challenge faced, and so too is the induced stress response. The eukaryote Saccharomyces cerevisiae is sensitive to low concentrations of the...
ORGANISM(S): Saccharomyces cerevisiae 
2014-02-13 | E-GEOD-54951 | biostudies-arrayexpress
MetabolomicsUntargeted metabolomics links glutathione to bacterial cell cycle progression
Cell cycle progression requires the coordination of cell growth, chromosome replication, and division. Consequently, a functional cell cycle must be coupled with metabolism. However, direct measurements of metabolome dynamics remained scarce, in particular in bacteria. Here, we describe an untargete...
2020-10-15 | MTBLS1402 | MetaboLights
TranscriptomicsS. cerevisiae time-course in the presence of 100uM BCS
Saccharomyces cerevisiae cells were exposed to a copper chelator (100uM BCS) for 48 hours. Samples were compared to the zero hour timepoint at 0.5, 1, 2, 3, 4, 24 and 48 hours.
ORGANISM(S): Saccharomyces cerevisiae 
2006-04-03 | E-UMCU-16 | biostudies-arrayexpress
ProteomicsS. cerevisiae subjected to prolonged heat stress of 37 ºC
Differential proteome composition of wild-type yeast cells (S. cerevisiae BY4741) grown at 30 ºC or 37 ºC was studied using SILAC based quantitative proteomics.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-04-23 | PXD001864 | Pride
Silac. heat stress. proteomics 
UnknownEvaluation of variable new antigen receptors (vNARs) as a novel cathepsin S (CTSS) targeting strategy.
Not available
S-EPMC10728302 | biostudies-literature
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