Sort   by:  
 Page size 
MetabolomicsCombining peak- and chromatogram-based retention time alignment algorithms for multiple chromatography-mass spectrometry datasets
Spring wheat (Triticum aestivum L.) was grown in a free-air carbon dioxide (CO2) enrichment (FACE) field experiment. Grain and biomass yield and its components were determined at maturity and the grain metabolome was analysed by gas chromatography-mass spectrometry (GC-MS) for elevated and ambient C...
2012-09-21 | MTBLS21 | MetaboLights
MetabolomicsNew plasma preparation approach to enrich metabolome coverage in untargeted metabolomics: plasma protein bound hydrophobic metabolite release with proteinase K
Plasma untargeted metabolomics is a common method for evaluation of the mechanisms underlying human pathologies and identification of novel biomarkers. The plasma proteins provide the environment for transport of hydrophobic metabolites. The current sample preparation protocol relies on the immediat...
2022-03-18 | MTBLS670 | MetaboLights
MetabolomicsCell Membrane Remodeling Mediates Polymyxin B Resistance in Klebsiella pneumoniae: An Integrated Proteomics and Metabolomics Study
Polymyxin B (PB) is introduced into the clinic as the last-line therapy against carbapenem-resistant Klebsiella pneumoniae (CRKP). Unfortunately, increased resistance to PB in Klebsiella pneumoniae (K. pneumoniae) has threatened global health. Resistance of K. pneumoniae ...
2022-03-04 | MTBLS4132 | MetaboLights
GenomicsK. lactis Ste12-myc ChIP-Seq in pheromone-responding a cells
The purpose of this experiment was to determine the genes directly regulated by Ste12 in K. lactis. The experiment was performed in a cells to determine if the a-specific genes were bound by Ste12. Ste12 was tagged with c-myc and was immunoprecipitated with a c-myc antibody. Cells were starved in SD...
ORGANISM(S): Kluyveromyces lactis 
2015-07-06 | E-GEOD-65792 | biostudies-arrayexpress
MetabolomicsIdentification of BolA protein reveals a novel virulence factor in Klebsiella pneumoniae that contributes to liver abscess in mice

BolA has been characterized as an important transcriptional regulator, which is induced in the stationary phase of growth and is often associated with bacterial virulence. This study was initiated to elucidate the role of the BolA in the virulence of K. pneumoniae. Using a mous...

2022-09-13 | MTBLS2712 | MetaboLights
ProteomicsDiscovery and design of molecular glue enhancers of CDK12-DDB1 interactions for targeted degradation of cyclin K
The CDK12 inhibitor, SR-4835, promotes the proteasomal degradation of cyclin K, contingent on the presence of CDK12 and the CUL4-RBX1-DDB1 E3 ligase complex. The inhibitor displays molecular glue activity, which correlates with its enhanced ability to inhibit cell growth. This effect is achieved by...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD053505 | Pride
Cdk12 Molecular glue degraders Cyclin k 
GenomicsThe DAXX co-repressor is directly recruited to active regulatory elements genome-wide to regulate autophagy programs in a model of human prostate cancer (ChIP-seq)
This study was aimed at understanding the genome-wide binding and regulatory role of the DAXX transcriptional repressor, recently implicated in PCa. ChIP-Seq analysis of genome-wide distribution of DAXX in PC3 cells revealed over 59,000 DAXX binding sites, found at regulatory enhancers and promoters...
ORGANISM(S): Homo sapiens 
2015-06-24 | E-GEOD-68647 | biostudies-arrayexpress
ProteomicsA cell-free strategy for profiling intracellular antibiotic sensitivity and resistance – characterising the proteomes of K. pneumoniae ATCC 13882, ST258-T1b and NJST258-1 and E. coli K-12 MG1655.
Antimicrobial resistance (AMR) is a pandemic spread across multiple infectious disease microbes. To provide a new tool to study AMR, here we develop a Klebsiella pneumoniae cell-free gene expression (CFE) system. To characterise the system, we use proteomics to compare this to a Escherichia coli MG1...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 Bacteria 
2023-11-29 | PXD046864 | Pride
Synthetic biology Cell-free protein synthesis Klebsiella pneumoniae Antimicrobial resistance Cell-free gene expression 
ProteomicsThe catalytic mechanism of vitamin K epoxide reduction in a cellular environment
Vitamin K epoxide reductases (VKOR) constitute a major family of integral membrane thiol oxidoreductases. In humans, VKOR sustains blood coagulation and bone mineralization through the vitamin K cycle. Previous chemical models assumed that the catalysis of human VKOR (hVKOR) starts from a fully redu...
ORGANISM(S): Homo sapiens (Human) 
2020-12-03 | PXD020676 | Pride
Human vkor Lc/ms/ms Catalytic mechanism 
ProteomicsIdentification of vitamin K-dependent proteins in mouse liver by LC-MS/MS
Vitamin K (VK), a fat-soluble vitamin, functions as a co-factor during the gamma-carboxylation reaction that converts glutamic acid residues to gamma-carboxyglutamic acid residues in proteins transiting through the endoplasmic reticulum. Two enzymes are involved which together form the VK cycle: gam...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-10 | PXD032920 | Pride
Gamma-carboxylation Mouse Liver Vitamin k Lc-ms/ms 
Sort   by:  
 Page size