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T cells from OT-I mice were stimulated with 4 different peptide ligands for 6 hours and sorted into two 96-well plates. Cells on each plate were barcoded using a mutually exclusive 8-by-12 set of indexes, such that indexes present on plate 1 were completely absent from plate 2 and vice versa. Librar...
ORGANISM(S): Mus musculus 
Virus-specific CD8 T cells from PBMCs of healthy donors and hepatitis patients were enriched for recognition of a panel of feature barcode-labeled pMHC multimers. The phenotypic description was made using gene expression and TCRseq. Samples were pooled and sequenced on the same 10x Genomics sequenci...
ORGANISM(S): Homo sapiens 
Effect of peptide IDR-1 on CD14+ human monocytes. >br< >br< Effect of peptide IDR-1 on LPS-induced responses in CD14+ human monocytes.>br< >br< Human peripheral blood mononuclear cells (PBMC) were treated with innate defence regulator peptide IDR-1 in the presence and absence of LPS (purified from P...
ORGANISM(S): Homo sapiens 
The objective of the study was to evaluate transcriptional response of endotoxin-stimulated human monocytic cells in presence and absence of host defense peptide LL-37. A functional genomics approach was used to establish a temporal transcriptional profile and identify differentially expressed gene...
ORGANISM(S): Homo sapiens 
Binding protein generation relies on laborious screening cascades that process candidate molecules individually. To break with this paradigm, we developed NestLink, a binder selection and identification technology able to biophysically characterize thousands of library members at once without handli...
ORGANISM(S): synthetic construct (code 12) 
2019-01-15 | PXD009301 | Pride
Early and accurate diagnosis Equine Gastric Ulcer Syndrome (EGUS) is critical for effective treatment and management. This study aimed to analyse the serum peptide barcode and peptidome profile of horses with EGUS using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and...
ORGANISM(S): Cellular Organisms 
microRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3’ untranslated region (UTR). However, it is unclear to what extent miRNAs ...
ORGANISM(S): Mus musculus 
DNA hydroxymethylation (5hmC) represents a new layer of epigenetic regulation in addition to DNA methylation (5mC). The genome-wide patterns of 5hmC distribution in many tissues and cells have recently been revealed by hydroxymethylated DNA immunoprecipitation (hMeDIP) followed by high throughput se...
ORGANISM(S): Mus musculus 
Single cell RNA-sequencing of T cells to examine barcode swapping
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