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ProteomicsCoupling microdroplet-based sample preparation, multiplexed isobaric labeling, and nanoflow peptide fractionation for deep proteome profiling of tissue microenvironment
Existing spatially-resolved proteomics technologies cannot provide deep proteome coverage due to limited sensitivity and poor sample recovery. Herein, we seamlessly combined laser capture microdissection with a low-volume sample processing technology that includes a microfluidic device named microPO...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-01-25 | MSV000093925 | MassIVE
ProteomicsPeptide N-terminal Bead Capture and Release
The field of proteomics has expanded recently with more sensitive techniques for the bulk measurement of peptides as well as single-molecule techniques. One limiting factor for these methods is the need for multiple chemical derivatizations and highly pure proteins free of contaminants. We show a so...
ORGANISM(S): Homo sapiens (Human) 
2021-06-01 | PXD016291 | Pride
Human N-terminal modification Lc-ms/ms 
ProteomicsPeptide N-terminal Bead Capture and Release
The field of proteomics has expanded recently with more sensitive techniques for the bulk measurement of peptides as well as single-molecule techniques. One limiting factor for these methods is the need for multiple chemical derivatizations and highly pure proteins free of contaminants. We show a so...
ORGANISM(S): Homo sapiens (Human) 
2020-09-16 | PXD017161 | Pride
Human N-terminal modification Lc-ms/ms 
ProteomicsProteomic profiling of centrosomes across multiple cell and tissue types by a new affinity capture method
Centrosomes are small cytoplasmic organelles that play fundamental roles in a range of cellular processes. Nearly all vertebrate cell types contain centrosomes, but whether centrosome composition and function differ between cell types, tissues and pathologies remains an outstanding question. Until n...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2023-10-18 | PXD040308 | Pride
Proteomics Capture peptide Capture-ms Centrosomes 
TranscriptomicsRapid isolation of extracellular vesicles from cell culture and biological fluids using a synthetic peptide with specific affinity for heat shock proteins
Background: Exosomes and extracellular vesicles (EVs) are increasingly recognized as important sources of biomarkers for disease study and diagnosis. Results: A synthetic peptide, Vn96, allows for capture of EVs from biological fluids using basic laboratory equipment. Conclusion: The Vn96-captured E...
ORGANISM(S): Homo sapiens 
2014-06-14 | E-GEOD-58464 | biostudies-arrayexpress
TranscriptomicsRNA-seq analysis of the fungal mycelium of the plant pathogen Pyricularia oryzae treated with and antimicrobial peptide analog of a Trichoderma peptaibol
We aimed at investigating the mode of action of an antimicrobial peptide analog of a Trichoderma peptaibol. Conidia of P. oryzae (IT10 strain) were cultured in Complete Medium at a final concentration of 2x105 conidia per mL for 3 days at 30 °C under shaking at 150 rpm. After three days, 50 µM of t...
ORGANISM(S): Pyricularia oryzae 70-15 
2021-10-08 | E-MTAB-10927 | biostudies-arrayexpress
TranscriptomicsRibosome profiling reveals pervasive and regulated stop codon readthrough in Drosophila melanogaster
Ribosomes can read through stop codons in a regulated manner, elongating rather than terminating the nascent peptide. Stop codon readthrough is essential to diverse viruses, and phylogenetically predicted to occur in a few hundred genes in Drosophila melanogaster, but the importance of regulated rea...
ORGANISM(S): Drosophila melanogaster 
2013-12-05 | E-GEOD-49197 | biostudies-arrayexpress
ProteomicsRNA interactome capture identifies key proteins for herpesviral gene expression
The primary mRNA sequence determines its secondary structure and the repertoire of interacting RNA binding proteins (RBPs). The resulting mRNA ribonucleoprotein complex (mRNP) then influences all stages of the life of an mRNA. Here, we determined the mRNP composition of individual Kaposi Sarcoma Her...
ORGANISM(S): Homo sapiens (Human) Human gammaherpesvirus 8 
2026-01-23 | PXD056253 | Pride
Kshv Au-rich sequences Srsf3 Rna-binding-proteins Rna interactome capture Rna stability Codon optimization Herpesviral gene expression Rbps Lytic transcripts Orf57 
ProteomicsAffinity peptide ligands: New tools for chasing non-canonical N-phosphoproteome
Protein N-phosphorylation enrichment is faced with great difficulties due to the intrinsic instability of the N-P bond, which has seriously hindered its biological function unraveling. Current methods for N-phosphopeptides enrichment are challenging to fulfil high selectivity for all types of N-phos...
ORGANISM(S): Homo Sapiens Escherichia Coli Mus Musculus 
2024-12-12 | PXD058797 |
TranscriptomicsRNA-Seq Analysis of Laser-Capture Microdissected Cells of the Developing Central Starchy Endosperm of Maize
Endosperm is a product of double fertilization, and provides nutrients and signals to the embryo during seed development in flowering plants. Early stages of endosperm development are critical for the development of its storage capacity through synthesis and accumulation of starch and storage protei...
ORGANISM(S): Zea mays 
2014-06-17 | E-GEOD-58504 | biostudies-arrayexpress
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