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Label the cells overexpressed Flag tagged YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 and SRSF10 with 4-SU, the RNA bound by YTHDC1 and SRSF proteins can be got by Flag IP followed by RNA isolation by using the TRIzol (Invitrogen) reagent by following the company manual.the RNA was then used for library prep...
ORGANISM(S): Homo sapiens 
RNA was isolated from and YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 and SRSF10 deficient human HeLa cells using the TRIzol (Invitrogen) reagent by following the company manual. For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. All samples showed a RIN (RNA integrity number) o...
ORGANISM(S): Homo sapiens 
Endogenous SRSF3 and SRSF4 RNA binding sites were identified genome-wide by iCLIP in P19 BAC trangenic cell lines using GFP-epitope tag.
ORGANISM(S): Mus musculus 
Alternative polyadenylation (APA) refers to the regulated selection of polyadenylation sites (PASs) in transcripts, which affects the length of their 3’ untranslated regions (3’UTRs). APA regulates stage- and tissue-specific gene expression by affecting the stability, subcellular localization or tra...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-09 | PXD018090 | Pride
This experiment aims to map SRSF3 binding to the global transcriptome in a mouse epicardial cell line
ORGANISM(S): Mus musculus 
RNA sequencing of SRSF3 depleted pluripotent cells
SRSF3 is a key regulator of epicardial formation
Cardiomyocyte-specific Srsf3 deletion reveals a mitochondrial regulatory role
RNA-Seq analysis of SRSF3 KO hearts compared to controls and iCLIP analysis of SRSF3 binding profile in neonatal cardiomyocytes
irCLIP of SRSF3 in a mouse epicardial cell line
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