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TranscriptomicsPAR-CLIP analysis of YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 and SRSF10 in human HeLa cells
Label the cells overexpressed Flag tagged YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 and SRSF10 with 4-SU, the RNA bound by YTHDC1 and SRSF proteins can be got by Flag IP followed by RNA isolation by using the TRIzol (Invitrogen) reagent by following the company manual.the RNA was then used for library prep...
ORGANISM(S): Homo sapiens 
2016-02-17 | E-GEOD-71096 | biostudies-arrayexpress
TranscriptomicsRNA seq of human breast cancer cell line MDAMB231 with knockdown of splicing factors SRSF7 and SMNDC1
Deep RNA sequencing was performed to investigate the transcriptomic effects of siRNA-mediated knockdown of SRSF7 or SMNDC1 in the triple-negative breast cancer cell line MDA-MB-231. A total of 30 million cells MDA-MB-231 cells were seeded in 6-well plates. The following day, cells were transfected w...
ORGANISM(S): Homo sapiens 
2026-06-01 | E-MTAB-16601 | biostudies-arrayexpress
GenomicsRIP-seq of SRSF7 in Huh7
RIP-seq of SRSF7 in Huh7
PRJNA863262 | ENA
TranscriptomicsTranscriptomics analysis of gene expression in normal and YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 or SRSF10 deficient human HeLa cells
RNA was isolated from and YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 and SRSF10 deficient human HeLa cells using the TRIzol (Invitrogen) reagent by following the company manual. For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. All samples showed a RIN (RNA integrity number) o...
ORGANISM(S): Homo sapiens 
2016-02-17 | E-GEOD-71095 | biostudies-arrayexpress
ProteomicsSRSF7 and SRSF3 affectSRSF7 and SRSF3 affect 3’UTR length in opposite directions by controlling proximal poly(A)-site usage and CFIm levels 3’UTR length in opposite directions by controlling proximal poly(A)-site usage and CFIm levels
Alternative polyadenylation (APA) refers to the regulated selection of polyadenylation sites (PASs) in transcripts, which affects the length of their 3’ untranslated regions (3’UTRs). APA regulates stage- and tissue-specific gene expression by affecting the stability, subcellular localization or tra...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-09 | PXD018090 | Pride
Mace Dapars Cfim Cpsf5 Srsf7 Cpsf6 Fip1 Srsf3 Poly(a) site Iclip Alternative polyadenylation 
ProteomicsPeptide mapping of SRSF7_RRM isoforms in murine P19 cells
Here we mapped peptides of mouse SRSF7_RRM purified from P19 cells.
ORGANISM(S): Mus musculus (Mouse) 
2020-05-27 | PXD016884 | Pride
Srsf7 Lc-msms 
GenomicsDecreased SRSF7 expression induces cellular senescence via generating MDM2 variants
Decreased SRSF7 expression induces cellular senescence via generating MDM2 variants
PRJNA961140 | ENA
ProteomicsPeptide mapping of SRSF7_RS-GFP isoforms in murine P19 cells
Here we mapped peptides of mouse SRSF7_RS-GFP purified from P19 cells.
ORGANISM(S): Mus musculus (Mouse) 
2020-05-27 | PXD016871 | Pride
Srsf7 Lc-msms 
UnknownSRSF7 maintains its homeostasis through the expression of Split-ORFs and nuclear body assembly.
Not available
S-EPMC7096898 | biostudies-literature
UnknownSRSF7 and SRSF3 depend on RNA sequencing motifs and secondary structures to regulate Microprocessor.
Not available
S-EPMC9905709 | biostudies-literature
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