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MetabolomicsBiosynthesis of Isonitrile Lipopeptides by Conserved Non-ribosomal Peptide Synthetase Gene Clusters in Actinobacteria
A putative lipopeptide biosynthetic gene cluster is conserved in many species of Actinobacteria, including Mycobacterium tuberculosis and Mycobacterium marinum, but the specific function of the encoding proteins has been elusive. Using both in vivo heterologous reconstitution and in vitro biochemica...
2017-07-18 | MTBLS454 | MetaboLights
GenomicsHigh Throughput pMHC-I Tetramer Library Production Using Chaperone Mediated Peptide Exchange
High Throughput pMHC-I Tetramer Library Production Using Chaperone Mediated Peptide Exchange
PRJNA612056 | ENA
ProteomicsMouse TAPBPR functions as an MHC-I peptide exchange catalyst
Human TAPBPR is known to function as a Major Histocompatibility Complex class I (MHC-I) peptide exchange catalyst which shapes the peptide repertoire presented to immune cells. Never14theless, investigations characterizing TAPBPR from other species are limited. Here, we characterize mouse TAPBPR, ex...
ORGANISM(S): Mus musculus (Mouse) 
2026-03-23 | PXD069965 | Pride
Mouse Tapbpr Immunopeptidomics Mhc-i Lc-ms/ms 
ProteomicsMouse TAPBPR functions as an MHC-I peptide exchange catalyst
Human TAPBPR is known to function as a Major Histocompatibility Complex class I (MHC-I) peptide exchange catalyst which shapes the peptide repertoire presented to immune cells. Never14theless, investigations characterizing TAPBPR from other species are limited. Here, we characterize mouse TAPBPR, ex...
ORGANISM(S): Mus musculus (Mouse) 
2026-03-23 | PXD068540 | Pride
H-2kb Mouse tapbpr Peptide exchange H-2db Mhc-i Calnexin 
OtherAnion-Exchange Chromatography of Phosphopeptides: Weak Anion Exchange versus Strong Anion Exchange and Anion-Exchange Chromatography versus Electrostatic Repulsion-Hydrophilic Interaction Chromatography.
Data from ProteomeXchange, PXD ID: PXD001333. File: Amanda ms1-8ppm ms2-20ppm 20130219_QEx3_RSLC-4_Mischerikow_Peters_IMP_shotgun_s2324.msf.mgf. Published as part of Anal Chem. 2015 Apr 17 . From the Abstract: {{i}} ... Weak anion-exchange (WAX) and strong anion-exchange (SAX) columns were compared...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32310019320 | GPMDB
Resource Reanalysis 
ProteomicsAnion-Exchange Chromatography of Tryptic and Phosphopeptides: WAX vs. SAX and AEX vs. ERLIC
At a pH > 5, phosphopeptides have two negative charges per residue and are well-retained in anion-exchange chromatography. However, the peptides with one or two phosphate groups are not separated from the peptides with multiple Asp or Glu residues, which interfere with the identification of phosph...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080774 | MassIVE
TranscriptomicsHigh Throughput pMHC-I Tetramer Library Production Using Chaperone Mediated Peptide Exchange
Peptide exchange technologies are essential for the generation of pMHC-multimer libraries for probing diverse, polyclonal TCR repertoires in various settings. Here we report, using the molecular chaperone TAPBPR, a robust method for the capture of stable, empty MHC-I molecules, which can be readily ...
ORGANISM(S): synthetic construct Homo sapiens 
2020-03-12 | GSE146826 | GEO
ProteomicsOne-step Isolation of Protein C-terminal Peptides from Protease-digested Proteins by Metal Oxide-based Ligand Exchange Chromatography
We have developed a one-step method to isolate protein C-terminal peptides from V8-digested proteins by metal oxide-based ligand-exchange (MOLEX) chromatography. V8 protease cleaves the C-terminal side of Asp and Glu, and the resulting digested peptide has two carboxy groups at the C-terminus, where...
ORGANISM(S): Homo Sapiens (human) 
2021-12-28 | PXD027960 | JPOST Repository
ProteomicsDynamics of an LPS translocon induced by substrate and an antimicrobial peptide
We investigated the conformational dynamics of the LPS translocon LptDE was using hydrogen-deuterium exchange mass spectrometry. We evaluated the conformational changes of LptDE upon binding of LPS, Re-LPS (an LPS substructure), thanatin, and the membrane lipid POPG. Our data reveal that LPS induces...
ORGANISM(S): Klebsiella pneumoniae 
2020-11-16 | PXD021743 | Pride
Lps Hydrogen-deuterium exchange Hdx-ms Thanatin Lptde 
ProteomicsTraceless peptide and protein modification via rational tuning of halopyridiniums
Building on the insights gained from systematically investigating of aromatic nucleophilic substitution (SNAr) and thiol-exchange, we identified the highly electron-deficient halopyridiniums as a tunable and predictable reaction platform for adjusting electrophilicity and thiol-cleavability by follo...
ORGANISM(S): Homo Sapiens 
2023-09-25 | PXD045683 |
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