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ProteomicsInfluence of digestion protocol in Protein Quantitation
Quantitative determination of absolute and relative protein amounts is an essential requirement for most current bottom-up proteomics applications, but protein quantitation estimates are affected by several sources of variability such as sample preparation, mass spectrometric acquisition, and data a...
ORGANISM(S): Escherichia coli 
2014-07-30 | PXD001187 | Pride
Protein quantitation Digestion protocols Fasp 
TranscriptomicsRNA sequencing of HEK293T cells expressing peptide 9J10, a 14-3-3 interacting peptide
We investigated the transcriptome profile of HEK293T cells transfected with a plasmid encoding 9J10, a peptide isolated in a phenotypic screen from a library of peptides derived from bacterial and archaeal genomes. The peptide was identified in a screen for FOXO3a reactivation and has been shown to...
ORGANISM(S): Homo sapiens 
2021-05-28 | E-MTAB-10161 | biostudies-arrayexpress
UnknownAlacatDesigner─Computational Design of Peptide Concatamers for Protein Quantitation.
Not available
S-EPMC9903321 | biostudies-literature
OtherInfluence of the Digestion Technique, Protease, and Missed Cleavage Peptides in Protein Quantitation.
Data from ProteomeXchange, PXD ID: PXD001187. Enzyme: LysC_Trypsin, file: 121219_S_CCES_01_03_LysC_Try_1to10_Mixt_1_3.mzXML. Published as part of J Proteome Res. 2014 Jul 28 . From the Abstract: {{i}} Here we evaluated both in-solution and filter-aided digestion protocols and assessed their influen...
ORGANISM(S): Enterobacteria_phage_13a_uid30603,enterobacteria_phage_933w_uid14043,enterobacteria_phage_alpha3_uid14570,enterobacteria_phage_ba14_uid30599,enterobacteria_phage_bp_4795_uid14287,enterobacteria_phage_bz13_uid14635,enterobacteria_phage_cdti_uid19737,enterobacteria_phage_ecods1_uid30601,enterobacteria_phage_eps7_uid29287,enterobacteria_phage_epsilon15_uid14285,enterobacteria_phage_es18_uid15174,enterobacteria_phage_felix_01_uid14323,enterobacteria_phage_fels_2_uid32273,enterobacteria_phage_fi_sensu_lato_uid15459,enterobacteria_phage_g4_sensu_lato_uid14318,enterobacteria_phage_hk022_uid14048,enterobacteria_phage_hk620_uid14115,enterobacteria_phage_hk97_uid14592,enterobacteria_phage_i2_2_uid14572,enterobacteria_phage_id18_sensu_lato_uid16628,enterobacteria_phage_id2_moscow_id_2001_uid16591,enterobacteria_phage_if1_uid14039,enterobacteria_phage_ike_uid14627,enterobacteria_phage_ime08_uid50177,enterobacteria_phage_jk06_uid15569,enterobacteria_phage_js10_uid38265,enterobacteria_phage_js98_uid27983,enterobacteria_phage_jse_uid38263,enterobacteria_phage_k1e_uid16228,enterobacteria_phage_k1f_uid15880,enterobacteria_phage_k1_5_uid17059,enterobacteria_phage_lambda_uid14204,enterobacteria_phage_m13_uid14549,enterobacteria_phage_min27_uid29143,enterobacteria_phage_ms2_uid14659,enterobacteria_phage_mu_uid14105,enterobacteria_phage_n15_uid14086,enterobacteria_phage_n4_uid18511,enterobacteria_phage_p1_uid14493,enterobacteria_phage_p22_uid14478,enterobacteria_phage_p2_uid14035,enterobacteria_phage_p4_uid14414,enterobacteria_phage_phi1_uid20789,enterobacteria_phage_phieco32_uid28729,enterobacteria_phage_phiecom_gj1_uid27979,enterobacteria_phage_phip27_uid14599,enterobacteria_phage_phiv10_uid16381,enterobacteria_phage_phix174_sensu_lato_uid14015,enterobacteria_phage_prd1_uid14062,enterobacteria_phage_psp3_uid14345,enterobacteria_phage_rb14_uid37825,enterobacteria_phage_rb16_uid51699,enterobacteria_phage_rb32_uid17997,enterobacteria_phage_rb43_uid15417,enterobacteria_phage_rb49_uid14301,enterobacteria_phage_rb51_uid37819,enterobacteria_phage_rb69_uid15141,enterobacteria_phage_rtp_uid16178,enterobacteria_phage_sf6_uid14498,enterobacteria_phage_sfv_uid14162,enterobacteria_phage_sp6_uid14291,enterobacteria_phage_ssl_2009a_uid34919,enterobacteria_phage_st104_uid14499,enterobacteria_phage_st64t_uid14230,enterobacteria_phage_st_1_uid38669,enterobacteria_phage_t1_uid14496,enterobacteria_phage_t3_uid14336,enterobacteria_phage_t4_uid14044,enterobacteria_phage_t5_uid15143,enterobacteria_phage_t7_uid14460,enterobacteria_phage_tls_uid19775,enterobacteria_phage_vt2_sakai_uid14480,enterobacteria_phage_wa13_sensu_lato_uid16595,enterobacteria_phage_wv8_uid38281,enterobacteria_phage_yyz_2008_uid32231,enterobacteriophage_qbeta_uid15479, Pxd001187, Escherichia_coli_k_12_substr__mg1655 
GPM32310002602 | GPMDB
Resource Reanalysis 
OtherOnline peptide fractionation using a multiphasic microfluidic LC chip improves reproducibility and detection limits for quantitation in discovery and targeted proteomics.
Data from ProteomeXchange, PXD ID: PXD001464. Experiment: 1hRP, file: CL_1hRP_rep4-Cell_Lysate_rep4.mgf. Published as part of Mol Cell Proteomics. 2015 Apr 7 . From the Abstract: {{i}} Comprehensive proteomic profiling of biological specimens usually requires multi-dimensional chromatographic pepti...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM11210037376 | GPMDB
Resource Reanalysis 
UnknownAbsolute Quantitation of Tryptophan-Containing Peptides and Amyloid β-Peptide Fragments by Coulometric Mass Spectrometry.
Not available
S-EPMC8925997 | biostudies-literature
UnknownSILAC peptide ratio calculator: a tool for SILAC quantitation of peptides and post-translational modifications.
Not available
S-EPMC3953147 | biostudies-literature
UnknowngpGrouper: A Peptide Grouping Algorithm for Gene-Centric Inference and Quantitation of Bottom-Up Proteomics Data.
Not available
S-EPMC6210220 | biostudies-literature
ProteomicsStadard peptide mixture plus HeLa
To test our peak detection and label-free quantitation algorithm, practical applications of AB3D for LC-MS data sets were evaluated using a standard peptide mixture consisting of bovine serum albumin (BSA) peptides with different concentrations, peptides derived from four standard proteins (data ...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2014-12-04 | PXD001259 | Pride
Lc-ms Peptide mixture 
UnknownPPINGUIN: Peptide Profiling Guided Identification of Proteins improves quantitation of iTRAQ ratios.
Not available
S-EPMC3368728 | biostudies-literature
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