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ProteomicsAnion-Exchange Chromatography of Tryptic and Phosphopeptides: WAX vs. SAX and AEX vs. ERLIC
At a pH > 5, phosphopeptides have two negative charges per residue and are well-retained in anion-exchange chromatography. However, the peptides with one or two phosphate groups are not separated from the peptides with multiple Asp or Glu residues, which interfere with the identification of phosph...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080774 | MassIVE
ProteomicsAnion-Exchange Chromatography of Tryptic and Phosphopeptides: WAX vs. SAX and AEX vs. ERLIC
At a pH > 5, phosphopeptides have two negative charges per residue and are well-retained in anion-exchange chromatography. However, the peptides with one or two phosphate groups are not separated from the peptides with multiple Asp or Glu residues, which interfere with the identification of phosph...
ORGANISM(S): Homo sapiens (Human) 
2015-04-23 | PXD001333 | Pride
Chromatography erlic sax wax aex 
UnknownNative CGRP Neuropeptide and Its Stable Analogue SAX, But Not CGRP Peptide Fragments, Inhibit Mucosal HIV-1 Transmission.
Not available
S-EPMC8692891 | biostudies-literature
ProteomicsLabel-Free Phosphoproteomics Profiling of Fission Yeast Schizosaccharomyces pombe
The phosphorylation of proteins modulates various functions of proteins and plays an important role in regulation of cell signaling. In the recent years, the label-free quantitative (LFQ) phos-phoproteomics has become the powerful tool to analyze the phosphorylation of proteins within the complex sa...
ORGANISM(S): Schizosaccharomyces pombe 927 
2021-02-12 | PXD023818 | Pride
Pgc Schizosaccharomyces pombe Sax Lfq phosphoproteomics 
ProteomicsEnhancing proteome coverage by using strong anion exchange in tandem with basic-pH reversed-phase chromatography for sample multiplexing-based proteomics
Sample multiplexing-based proteomic strategies rely on fractionation to improve proteome coverage. Tandem mass tag (TMT) experiments, for example, can currently accommodate up to 18 samples with proteins spanning several orders of magnitude, thus necessitating fractionation to achieve reasonable pro...
ORGANISM(S): Homo sapiens (Human) 
2023-12-28 | PXD044393 | Pride
Faims Tmtpro Eclipse Sax Sps-ms3 Rts 
ProteomicsComparison of Intact Glycopeptide Enrichment Methods Using Strong Anion Exchange and Hydrophilic Interaction Liquid Chromatography
Heterogeneity of protein glycosylation poses great challenge for analysis that is key to un-puzzle systems glycobiology in diseases. Resolving this conundrum requires global enrichment of glycopeptides for identification and quantitation. To this aim, hydrophilic interaction chromatography (HILIC) h...
ORGANISM(S): Homo sapiens (Human) 
2017-10-19 | PXD005145 | Pride
Glycoproteomics Hilic Sax Quantitation O-linked 
OtherAnion-Exchange Chromatography of Phosphopeptides: Weak Anion Exchange versus Strong Anion Exchange and Anion-Exchange Chromatography versus Electrostatic Repulsion-Hydrophilic Interaction Chromatography.
Data from ProteomeXchange, PXD ID: PXD001333. File: Amanda ms1-8ppm ms2-20ppm 20130219_QEx3_RSLC-4_Mischerikow_Peters_IMP_shotgun_s2324.msf.mgf. Published as part of Anal Chem. 2015 Apr 17 . From the Abstract: {{i}} ... Weak anion-exchange (WAX) and strong anion-exchange (SAX) columns were compared...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32310019320 | GPMDB
Resource Reanalysis 
GenomicsIdentification of Transcription Factor ZK377.2::GFP Binding Regions in L2
modENCODE_submission_3591 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is ta...
ORGANISM(S): Caenorhabditis elegans 
2012-05-09 | E-GEOD-37879 | biostudies-arrayexpress
GenomicsIdentification of Transcription Factor ZK377.2::GFP Binding Regions in L1
modENCODE_submission_3590 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is ta...
ORGANISM(S): Caenorhabditis elegans 
2013-02-04 | E-GEOD-44009 | biostudies-arrayexpress
GenomicsIdentification of Transcription Factor ZK377.2::GFP Binding Regions in L3
modENCODE_submission_3592 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is ta...
ORGANISM(S): Caenorhabditis elegans 
2013-02-04 | E-GEOD-44010 | biostudies-arrayexpress
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