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We introduce pupylation-based proximity labelling (PUP-IT) as a tool for protein interaction detection in plant biology. We show that PUP-IT readily confirmed and extended protein interactions for several known protein complexes, across different types of plant systems.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Nicotiana tabacum (Common tobacco) 
2025-01-10 | PXD048346 | Pride
Salt stress is one of the most detrimental abiotic stresses for plants. The plant cell wall, which encases plant cells and functions as a cellular exoskeleton, is an important structure to cope with such stresses. One of the main components of the cell wall is cellulose, which is synthesized by cell...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-08-30 | PXD047667 | Pride
Adjacent plant cells are connected by specialized cell wall regions, called middle lamellae, which influence critical agricultural characteristics, including fruit ripening and organ abscission. Middle lamellae are enriched in pectin polysaccharides, specifically homogalacturonan (HG). Pectins are m...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-03-20 | PXD047727 | Pride
To understand how an inhibition of the mitochondrial ATP synthase affects transcriptional programming and to identify potential candidates of the signaling machinery involved in ATP synthase deficiency responses, we used oligomycin on seedling liquid cultures. Seedlings were harvested at time points...
ORGANISM(S): Arabidopsis thaliana 
In this study, we use Cross-linking Mass Spectrometry to identify the interaction sites of the microtubule binding N-terminal domain of the companion of cellulose synthase 1 protein (CC1∆C223) from Arabidopsis thaliana with tubulin. We consistently detected four cross-linked peptides of CC1∆C223 to ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-01-08 | PXD009260 | Pride
STO2 is a novel MYB like protein which belongs to one of the most important transcription factors in planta. Microarray analysis of sto2 null mutant compared to Columbia with three-week-old soil-grown plants revealed that STO2 function in ABA and salt stress response. To find the potantial downstrea...
ORGANISM(S): Arabidopsis thaliana 
Expression profling of Arabidopsis sto2 mutant
Plants typically contain two different types of cell walls: a primary wall that is being deposited around all growing cells, and a secondary wall that is produced in cells with specialized functions once they have ceased to grow. In Arabidopsis, VND7 is a transcription factor that is sufficient to a...
ORGANISM(S): Arabidopsis thaliana 
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