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Gene expression is controlled under spatial chromatin structures with short-range in topologically associating domains (TAD) and long-range chromatin interactions between TADs, compartments or chromosomes, and disruption of chromatin structure leads to human diseases. The mechanism of short-range ch...
ORGANISM(S): Mus musculus 
2022-08-10 | GSE147919 | GEO
Manipulation of Long-Range Chromatin Interactions through CTCF Phase Separation for Cell Fate Control
Lameness due to femoral head separation (FHS) is a production and welfare issue in commercial poultry. FHS is an idiopathic disorder, which is attributed to a myriad of factors but in order to improve bone health, broiler breeders must be prognosed for disease susceptibility and selected against FHS...
ORGANISM(S): Gallus gallus (Chicken) 
2016-08-09 | PXD003292 | Pride
total lipid extracts in solvent mixture containing acetonitrile, water, and isopropanol (ACN:H2O:IPA, 5:4:1, v/v/v). Seperation using UHPLC Ultimate 3000 system with 5 uL of sample injection onto an Accucore C30 column (2.1 mm * 150 mm, 2.6-um particle size) maintained at 40C. flow rate was set to...
ORGANISM(S): Environmental Samples <bacillariophyta> (ncbitaxon:33858) 
2024-10-19 | MSV000096136 | MassIVE
Our results show that CLK2 undergoes liquid-liquid phase separation (LLPS) in response to heat shock stress. Phosphorylation of CLK2 at T343 prevents the LLPS of CLK2. To identify the proteins that recruited to the CLK2 condensates, we immunoprecipitated CLK2 (WT) or (T343A) proteins with FLAG antib...
ORGANISM(S): Homo sapiens (Human) 
2023-11-06 | PXD040997 | Pride
Heat stress inhibits photosynthesis efficiency, thereby suppressing plant growth and crop yield. However, the mechanism underlying this inhibition is not fully understood. Here, we report that the multiple organellar RNA-editing factor 8 (MORF8) forms condensates with solid-like properties in chloro...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-03-07 | PXD061133 | Pride
Human articular chondrocytes were isolated from normal or osteoarthritic tissue. RNA decay was measured across the transcriptome in these cells by microarray analysis following an actinomycin D chase for 0, 1, 3 and 5 hours. Normalisation was conducted by quantile normalising each set of four decay ...
ORGANISM(S): Homo sapiens 
We established a rat model of myocardial Infarction by performing a surgical operation of left anterior descending coronary artery occlusion under sterie conditions. Wistar rats were previously randomly divided into 4 groups: control, ischemia, ischemia-propranolol, and non-ischemia-propranolol. Our...
ORGANISM(S): Rattus norvegicus 
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