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ProteomicsAccurate MS-based Rab10 phosphorylation stoichiometry determination as readout for LRRK2 activity in Parkinson’s disease
Pathogenic mutations in the Leucine-rich repeat kinase 2 (LRRK2) are the predominant genetic cause of Parkinson’s disease (PD). They increase its activity, resulting in augmented Rab10-Thr73 phosphorylation and conversely, LRRK2 inhibition decreases pRab10 levels. Currently, there is no assay to qua...
ORGANISM(S): Homo sapiens (Human) 
2020-07-03 | PXD019814 | Pride
Phosphorylation stoichiometry Targeted mass spectrometry Parkinson’s disease 
ProteomicsAccurate MS-based Rab10 phosphorylation stoichiometry determination as readout for LRRK2 activity in Parkinson’s disease
Pathogenic mutations in the Leucine-rich repeat kinase 2 (LRRK2) are the predominant genetic cause of Parkinson’s disease (PD). They increase its activity, resulting in augmented Rab10-Thr73 phosphorylation and conversely, LRRK2 inhibition decreases pRab10 levels. Monitoring pRab10 can thus serve as...
ORGANISM(S): Homo sapiens (Human) 
2020-07-03 | PXD015219 | Pride
Parkinson's disease Phosphoproteomics 
UnknownAccurate MS-based Rab10 Phosphorylation Stoichiometry Determination as Readout for LRRK2 Activity in Parkinson's Disease.
Not available
S-EPMC8143643 | biostudies-literature
ProteomicsIntegrated lysine acetylation stoichiometry and proteomics analysis in human cells
Lysine acetylation is a widespread posttranslational modification that targets a large number of biological pathways. Recent studies reveal that lysine acetylation sites exhibit mainly low stoichiometry. Here we explored three sample preparation methods, the use of detergents for the chemical acetyl...
ORGANISM(S): Homo sapiens (Human) 
2017-09-12 | PXD005903 | Pride
Deacetylase inhibitor Sirt1 Cancer cells Lysine acetylation Stoichiometry Proteomics 
UnknownImproved Method for Determining Absolute Phosphorylation Stoichiometry Using Bayesian Statistics and Isobaric Labeling.
Not available
S-EPMC6301010 | biostudies-literature
ProteomicsLarge-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics
We developed a motif-based isotopic quantitative method for determination of phosphorylation stoichiometry based on kinase selection.
ORGANISM(S): Homo sapiens (Human) 
2015-03-30 | PXD000728 | Pride
Motif Imac Drug resistance Phosphorylation stoichiometry Lung cancer 
ProteomicsDifferential stoichiometry among core ribosomal proteins
Understanding the regulation and structure of ribosomes is essential to understanding protein synthesis and its dysregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribosomal proteins (RPs), some experiments suggest a more variable composition. Testi...
ORGANISM(S): Saccharomycetales (ncbitaxon:4892) Mus Musculus (ncbitaxon:10090) 
2015-08-28 | MSV000079280 | MassIVE
UnknownProbing the stoichiometry of β2-adrenergic receptor phosphorylation by targeted mass spectrometry.
Not available
S-EPMC4022239 | biostudies-literature
UnknownStable isotope-free relative and absolute quantitation of protein phosphorylation stoichiometry by MS.
Not available
S-EPMC552780 | biostudies-literature
ProteomicsAcetylation Dynamics and Stoichiometry in Saccharomyces cerevisiae
Quantitative MS analysis of acetylation in yeast using SILAC labeling and MaxQuant. Download Index of Raw files first. We used quantitative mass spectrometry to analyze acetylation dynamics and stoichiometry in Saccharomyces cerevisiae. We found that acetylation accumulated in growth-arrested...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2014-02-03 | PXD000507 | Pride
Acetylation Metabolism Yeast Mitochondria Stoichiometry 
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