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GenomicsChemotranscriptomic Profiling with a Thiamine Monophosphate Photoaffinity Probe
Chemotranscriptomic Profiling with a Thiamine Monophosphate Photoaffinity Probe
PRJNA1160294 | ENA
ProteomicsProteome-wide profiling of olaparib interactors using a biotinylated photoaffinity probe
Olaparib is a widely used PARP inhibitor for the treatment of BRCA-mutated cancers. To comprehensively understand the drug's clinical impact, measuring its interactions with intended on- and off-targets is crucial. In this study, olaparib's on- and off-targets were profiled using photoaffinity label...
ORGANISM(S): Homo sapiens (Human) 
2025-02-04 | PXD060372 | Pride
Parp inhibitors Photoaffinity labeling Chemical proteomics Olaparib 
TranscriptomicsChemotranscriptomic Profiling with a Thiamine Monophosphate Photoaffinity Probe
RNA is a multifaceted biomolecule with numerous biological functions and can interact with small molecule metabolites as exemplified by riboswitches. Here, we profile the Escherichia Coli transcriptome on interactions with the metabolite Thiamine Monophosphate (TMP). We designed and synthesized a ph...
ORGANISM(S): Escherichia coli 
2025-02-17 | GSE277048 | GEO
ProteomicsSelectivity profiling of SLC16A3/MCT4 inhibitor slCeMM1 using photoaffinity probe
To assess the selectivity of SLC16A3/MCT4 inhibitor slCeMM1, we derived diazirine-alkyne photoaffinity probe (slCeMM1-PAP). We next used HAP1 and MDAMB231 cell lines for MS experiment, comparing the enrichment of proteins by slCeMM1-PAP and conditions where slCeMM1-PAP was competed with slCeMM1 or i...
ORGANISM(S): Homo sapiens (Human) 
2023-10-16 | PXD040089 | Pride
Photoaffinity probe  ap-ms  tmt  chemoproteomics 
ProteomicsDevelopment of nucleus-targeted synthetic histone photoaffinity probes
Dissection of the physiological interactomes of histone post-translational modifications (hPTMs) is crucial for understanding fundamental epigenetic regulatory pathways. Current peptide- or protein-based histone photoaffinity probes have greatly expanded the ability to probe the epigenetic interacto...
ORGANISM(S): Mus musculus (Mouse) 
2024-11-21 | PXD045620 | Pride
Nucleus-targeted histone photoaffinity probe Lactylation The epigenetic interactome Hard-to-transfect cells Native cells 
ProteomicsSulfoxide-diazirine (SODA) as a cleavable photoaffinity group to enable identification of photolabeled modification sites via LC-MS3
Photoaffinity labeling is a common approach in chemical proteomics for identification of small molecule probe tar-gets. However, the modification site on the target protein remains difficult to identify, because of the probe modifica-tion and potential fragmentation during tandem mass spectrometry. ...
ORGANISM(S): Bos taurus (Bovine) 
2025-08-25 | PXD065837 | Pride
Sulfoxide-diazirine Photolabeleing Lc-ms3 Cleavable photoaffinity group 
Proteomicsphotoaffinity for Puerarin and mouse brainstem
To elucidate the molecular mechanism mediating the inactivated effect of DMV neurons on fat absorption, we performed an activity-based protein profiling strategy, using Puerarin as a “bait”. The Puerarin-tag probe was synthesized with a photoreactive tag to enrich and visualize target proteins via a...
ORGANISM(S): Mus musculus (Mouse) 
2024-09-06 | PXD052140 | Pride
Puerarin brainstem 
GenomicsComparative Analysis of Alkyne- and Desthiobiotinylated Photoaffinity Probes for Chemotranscriptomic Profiling
Comparative Analysis of Alkyne- and Desthiobiotinylated Photoaffinity Probes for Chemotranscriptomic Profiling
PRJNA1416391 | ENA
ProteomicsSoDA-PAL Protein Profiling Proteomics of Sorgoleone Catabolizing Microbial Isolate Acinetobacter pittii SO1
Photoaffinity probe-based protein labeling of microbial isolate Acinetobacter pittii SO1 (NCBITAXON:48296), isolated from the sorghum rhizosphere that can utilize sorgoleone as a sole carbon source in order to identify proteins that bind sorgoleone. Photoaffinity labeling of A. pittii lysates using ...
ORGANISM(S): Acinetobacter Pittii (ncbitaxon:48296) 
2023-05-18 | MSV000091986 | MassIVE
UnknownA clickable photoaffinity probe of betulinic acid identifies tropomyosin as a target.
Not available
S-EPMC9136574 | biostudies-literature
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