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ProteomicsDetection of structure-specific protein–protein interactions of alpha-synuclein
Parkinson’s disease (PD) is a neurodegenerative disorder associated with the accumulation of intraneuronal protein aggregates called Lewy bodies. These inclusions contain aberrantly folded and aggregated alpha-synuclein (aSyn), a key protein involved in the pathology of PD, for which the mechanisms ...
ORGANISM(S): Homo sapiens (Human) 
2024-02-10 | PXD039784 | Pride
Amyloid fibrils Neurodegeneration Structural proteomics Protein-protein interactions Lc-msms Alpha-synuclein Interactome Induced pluripotent stem cell-derived cortical neurons Mass spectrometry Limited proteolysis Parkinson’s disease Proteomics Structure-specific interactions 
ProteomicsProbing antigen–antibody interactions using limited proteolysis–mass spectrometry
Protein–protein interactions (PPIs) mediate multiple essential functions and regulatory events in living organisms. The physical interactome of a given protein can be abnormally altered in response to external and internal cues, thus modulating cell physiology and contributing to human pathologies i...
ORGANISM(S): Homo sapiens (Human) 
2024-02-10 | PXD039481 | Pride
Lc-msms Antibody-antigen interaction Interactome Mass spectrometry Respiratory syncytial virus fusion glycoprotein Limited proteolysis Structural proteomics Protein-protein interactions Mammalian cells Proteomics 
TranscriptomicsExpression data from wild-type FY4 and GCR2 deletion strain
Expression data from wild-type FY4 and GCR2 deletion strain. Impact of the transcription factor Gcr2p on mRNA expression was investigated in the corresponding deletion strain in exponentially growing glucose minimal medium batch cultures. Both streins were grown on glucose minimal medium and harvest...
ORGANISM(S): Saccharomyces cerevisiae 
2010-03-06 | E-GEOD-19569 | biostudies-arrayexpress
ProteomicsLimited proteolysis–mass spectrometry detects protein–protein interactions of integral membrane proteins
Integral membrane proteins (IMPs) are permanently embedded within the plasma membrane and execute multiple cellular functions. IMPs constitute important targets, but development of drugs that target IMPs to modulate protein–protein interactions, which mediate their functions, has been challenging. C...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2024-02-10 | PXD039520 | Pride
Lc-msms Membrane proteins Interactome Mass spectrometry Limited proteolysis Structural proteomics Protein-protein interactions Proteomics Crude membranes 
ProteomicsAlpha-synuclein interacts with regulators of ATP homeostasis in mitochondria
Mitochondrial dysfunction and accumulation of α-synuclein aggregates are hallmarks of the neurodegenerative Parkinson’s disease and may be interconnected. In order to investigate the interplay between α-synuclein and brain mitochondria at near atomic structural level we applied NMR and identified α-...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2025-07-18 | PXD063500 | Pride
Lip-ms Mitochondria Alpha-synuclein 
ProteomicsLimited proteolysis-coupled mass spectrometry captures proteome-wide protein structural alterations and biomolecular condensation in living cells
To study protein structures directly within cells, we developed in-cell limited proteolysis-coupled mass spectrometry (in-cell LiP-MS). Conditions for introduction of proteinase K into human cells using electroporation were optimized and validated for intracellular cleavages. In-cell LiP-MS captured...
ORGANISM(S): Homo sapiens (Human) 
2025-11-09 | PXD069095 | Pride
Stress granules Mass spectrometry In-cell lip Structural proteomics Limited proteolysis Arsenite treatment Rapamycin 
UnknownSystematic identification of 20S proteasome substrates.
Not available
S-EPMC10987551 | biostudies-literature
ProteomicsDetection of structure-specific interactions of Rab GTPases
Protein–protein interactions (PPIs) mediate multiple essential functions and regulatory events in living organisms. The physical interactome of a given protein can be abnormally altered in response to external and internal cues, thus modulating cell physiology and contributing to human pathologies i...
ORGANISM(S): Homo sapiens (Human) 
2024-02-10 | PXD048849 | Pride
Lc-msms Interactome Mass spectrometry Limited proteolysis Structural proteomics Protein-protein interactions Proteomics Rab gtpases Structure-specific interactions 
UnknownGlobal profiling of protein complex dynamics with an experimental library of protein interaction markers.
Not available
S-EPMC12440823 | biostudies-literature
ProteomicsIn situ analysis of osmolyte mechanisms of proteome thermal stabilisation
Organisms across all kingdoms of life use small organic molecules called osmolytes to adapt to diverse environmental conditions and preserve viability. Osmolytes are thought to thermally stabilise proteins based on in vitro studies of a few purified proteins. The molecular mechanisms of osmolytes re...
ORGANISM(S): Escherichia coli 
2024-08-09 | PXD036186 | Pride
Osmolyte mechanisms Structural proteomics Stress adaptation Thermal stabilisation Aggregation 
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