Sperm isolated from WT mice and PITHD1-deficient mice in triplicate were lysed in 6 M guanidine-HCl containing 100 mM Tris-HCl, pH 8.0, and 2 mM DTT. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 × g for 15 min at 4 °C. The supernatants containing 100 µg ...
ORGANISM(S): Mus Musculus (mouse)
