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TranscriptomicsRNA-Immunoprecipitation of PIWIL1/piRNA complexes in COLO 205 cells and deep-sequencing of associated small RNAs with NGS technologies
To evaluate PIWIL1/piRNAs association in the metastatic COLO 205 cell line, we performed an RNA-immunoprecipitation experiment followed by small RNA deep-sequencing (RIP-Seq). For the identification of PIWIL1/piRNA complexes, independent reactions were carried out with two different antibodies (IP1 ...
ORGANISM(S): Homo sapiens 
2020-08-05 | E-MTAB-8121 | biostudies-arrayexpress
GenomicsPiwil1 Regulates Glioma Stem Cell Maintenance and Glioblastoma Progression
Piwil1 Regulates Glioma Stem Cell Maintenance and Glioblastoma Progression
PRJNA673195 | ENA
ProteomicsAnalysis of Global Protein Expression in PIWIL1 knockdown PDAC cell line
In contrast to the great advances in understanding of the functions of Piwi in germlines, their actions in cancer cells remain less clear. In particular, Piwi proteins have been asserted as oncoproteins, but little is known about how their oncogenic function is achieved in cancer cells. We conducted...
ORGANISM(S): Homo sapiens (Human) 
2020-02-18 | PXD017452 | Pride
Human Piwil1 Pdac 
TranscriptomicsArray-based DNA methylation profiling in male infertility reveals allele-specific DNA methylation in PIWIL1 and PIWIL2
Array-based DNA methylation profiling in peripheral blood leukocytes of 30 infertile men with impaired spermatogenesis as compared to 10 fertile men using the Illumina Infinium HumanMethylation 450k Bead Chip reveald 471 CpG sites (287 genes) to be differentially methylated between both groups. Thes...
ORGANISM(S): Homo sapiens 
2014-02-16 | E-GEOD-51245 | biostudies-arrayexpress
TranscriptomicsPiwil1 Regulates Glioma Stem Cell Maintenance and Glioblastoma Progression
Piwi proteins are a subfamily of Argonaute proteins that maintain germ cells in eukaryotes. However, the role of their human homologues in cancer stem cells and more broadly in cancer is poorly understood. Here, we report that the Piwi-like family members, including Piwil1 (Hiwi), are overexpressed ...
ORGANISM(S): Homo sapiens 
2020-10-30 | GSE160437 | GEO
UnknownPiwil1 Regulates Glioma Stem Cell Maintenance and Glioblastoma Progression.
Not available
S-EPMC7837390 | biostudies-literature
UnknownPIWIL1 promotes gastric cancer via a piRNA-independent mechanism.
Not available
S-EPMC7486755 | biostudies-literature
UnknownMeioc-Piwil1 complexes regulate rRNA transcription for differentiation of spermatogonial stem cells.
Not available
S-EPMC12289311 | biostudies-literature
UnknownMeioc-Piwil1 complexes regulate rRNA transcription for differentiation of spermatogonial stem cells.
Not available
S-EPMC11601514 | biostudies-literature
TranscriptomicsMouse adult testes: Total RNA vs. IP RNA
RNAs that are enriched in AGO2 Immunoprecipitated (IP) products or PIWIL1 IP products were identified from mouse(BALB/C) adult testes by examine the ratio of total RNA signal intensity to AGO2 IP RNA or PIWIL1 IP RNA signal intensity. Two-condition experiment,Total RNA extracted from mouse adult tes...
ORGANISM(S): Mus musculus 
2013-04-01 | E-GEOD-27582 | biostudies-arrayexpress
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