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We employed chromatin pull-down and deep sequencing to globally identify HetR DNA targets in vivo at 6 hours after fixed-nitrogen deprivation. We identified novel DNA binding targets of tagged HetR-6xHis and defined a consensus HetR binding site from these HetR target sequences. Chromatin pull down ...
ORGANISM(S): Nostoc sp. PCC 7120 
In this study, pull down assays combined with mass spectrometry analysis were performed in HL-1 cardiomyocytes transfected with control plasmid (OE-Vector) or LOC107984012-overexpression plasmid to identify the repertoire of LOC107984012 interacting proteins.
ORGANISM(S): Mus musculus (Mouse) 
2024-01-26 | PXD036966 | Pride
RNA pull-down analysis of hsa_circ_0001400 in 293T cells
In this study, pull down assays combined with mass spectrometry analysis were performed in AC16 cardiomyocytes transfected with control plasmid (OE-Vector) or LOC107984012-overexpression plasmid to identify the repertoire of LOC107984012 interacting proteins. Pierce™ Magnetic RNA-Protein Pull-Down K...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD037001 | Pride
To further investigate the transcription cofactor of Smad3 that regulated the transcription of PRDM16, we conducted DNA pull-down assay. we treated HK-2 cells with TGF-β and extracted the nuclear proteins. And the nuclear proteins were incubated with streptavidin magnetic beads, and biotin-labeled D...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD044753 | Pride
Recent studies have revealed the importance of long noncoding RNAs (lncRNAs) as tissue-specific regulators of gene expression. There is ample evidence that distinct types of vasculature undergo tight transcriptional control to preserve their structure, identity, and functions. We determined, for the...
ORGANISM(S): Homo sapiens (Human) 
2020-12-21 | PXD018578 | Pride
Nucleoid-associated proteins (NAPs) are known to fold bacterial DNA and influence global transcription. Incompatibility P-7 plasmid pCAR1 carries three genes encoding NAPs: H-NS family protein Pmr, NdpA-like protein Pnd, and HU-like protein Phu. Because previous reports about plasmid-encoded NAPs ma...
ORGANISM(S): Pseudomonas putida Pseudomonas 
2013-12-07 | GSE53068 | GEO
Nucleoid-associated proteins (NAPs) are known to fold bacterial DNA and influence global transcription. Incompatibility P-7 plasmid pCAR1 carries three genes encoding NAPs: H-NS family protein Pmr, NdpA-like protein Pnd, and HU-like protein Phu. Because previous reports about plasmid-encoded NAPs ma...
ORGANISM(S): Pseudomonas putida 
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