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ProteomicsNAGK sulfonylation data associated with "A lysis-derived PMSF and orthovanadate oxidant defines a distinct cysteine-engagement profile"
The N-acetylglucosamine kinase, NAGK, was found to undergo a mobility shift on SDS-PAGE that was reliant on the presence of PMSF and orthovanadate in the lysis buffer. The mobility shift was prevented by the addition of reducing agent to the lysis buffer, so in the assay described and deposited here...
ORGANISM(S): Homo sapiens (Human) 
2026-08-08 | PXD081578 | Pride
Nagk Pmsf Sulfonylation Orthovanadate 
ProteomicsPMSF/vanadate oxidation effect on Human PANC-1 cells
Cysteines play critical roles in redox regulation and can be covalently engaged by electrophiles to inform on cysteine function and targetability. Here, we compare cysteine engagement by a PMSF/orthovanadate-derived oxidant with that of pervanadate, another vanadium-based oxidant.
ORGANISM(S): Homo sapiens (Human) 
2026-08-07 | PXD076162 | Pride
UnknownProlyl Endopeptidase-Like Facilitates the α-Synuclein Aggregation Seeding, and This Effect Is Reverted by Serine Peptidase Inhibitor PMSF.
Not available
S-EPMC7355856 | biostudies-literature
TranscriptomicsTranscription profiling of Trichoderma reesei QM6a to assess the cellular effects of expression of misfolded CBHI molecules
Cultures were grown at 28 oC for 48 h on a shaker at 250 rpm. MG132 (60 ï¾µM, final concentration) in phenylmethylsulfonyl fluoride (PMSF) was added after 46 h or PMSF was added alone to the cultures without MG132. Following sample collection, mycelia were washed with 0.9 % (w/v) NaCl in RNAse-free ...
ORGANISM(S): Trichoderma reesei QM6a 
2009-05-29 | E-MEXP-2190 | biostudies-arrayexpress
Proteomicsproximity labeling 4T1 Cells through PCN
The 4T1 cells were labeled with BxxP via HA-PCN nanozyme as described above. After washing 5 times with PBS, cells were collected and lysed in RIPA buffer containing 1 % (v/v) Protease inhibitor cocktail (ThermoFisher Scientific) and 1 % (v/v) PMSF under bath-sonication, followed by centrifugation f...
ORGANISM(S): Mus musculus (Mouse) 
2025-07-29 | PXD057009 | Pride
4t1 pcn 
UnknownAddition of Phenylmethylsulfonyl Fluoride Increases the Working Lifetime of the Trout Liver S9 Substrate Depletion Assay, Resulting in Improved Detection of Low Intrinsic Clearance Rates.
Not available
S-EPMC7901806 | biostudies-literature
UnknownEffects of arachidonyl-2'-chloroethylamide (ACEA) on the protective action of various antiepileptic drugs in the 6-Hz corneal stimulation model in mice.
Not available
S-EPMC5578658 | biostudies-literature
UnknownInteraction of Human Drug-Metabolizing CYP3A4 with Small Inhibitory Molecules.
Not available
S-EPMC6448587 | biostudies-literature
UnknownNeutrophil cathepsin G, but not elastase, induces aggregation of MCF-7 mammary carcinoma cells by a protease activity-dependent cell-oriented mechanism.
Not available
S-EPMC3996324 | biostudies-literature
UnknownPhenylmethanesulphonyl fluoride inhibits GPI anchor biosynthesis in the African trypanosome.
Not available
S-EPMC452885 | biostudies-literature
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