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TranscriptomicsTranscriptional gene silencing mediated by interplay of MOM1 and Pol V
In plants, multiple levels of epigenetic control suppress transposon movement and permit somatic as well as transgenerational transmission of gene expression patterns. A variety of factors are involved that establish and/or maintain epigenetic marks, such as covalent modification of DNA and histones...
ORGANISM(S): Arabidopsis thaliana 
2010-06-19 | E-GEOD-17940 | biostudies-arrayexpress
TranscriptomicsEpigenetic memory mediated by HDA6, Pol IV and Pol V in Arabidopsis.
Multiple RNA-sequencing datasets were generated from wild-type Col-0 and hda6, pol IV or pol V null mutant samples to dissect genetic requirements for the maintenance of epigenetic memory and silent locus identity in Arabidopsis thaliana.
ORGANISM(S): Arabidopsis thaliana 
2014-03-27 | E-MTAB-4337 | biostudies-arrayexpress
ProteomicsAffinity purified epitope-taggedPol V LC-MSMS
The full mass spectrometry report of affinity purified epitope-tagged Pol V from A. thiniana T87 cells.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-07-20 | PXD041996 | Pride
T87 Pol v 
TranscriptomicsThe DDR complex facilitates the genome-wide association of RNA Polymerase V to promoters and evolutionarily young transposons
The plant-specific DNA-dependent RNA polymerase V (Pol V) evolved from Pol II to function in an RNA-directed DNA methylation pathway. Here, we have identified targets of Pol V in Arabidopsis thaliana on a genome-wide scale using ChIP-seq of NRPE1, the largest catalytic subunit of Pol V. We found tha...
ORGANISM(S): Arabidopsis thaliana 
2012-08-05 | E-GEOD-39247 | biostudies-arrayexpress
TranscriptomicsProfiling of RNA Pol II occupancy in Drosophila neural stem cell populations using TaDa (Targeted DamID)
Cell-type specific transcriptional profiling is key to understanding cell fate specification and function. In order to achieve this it has been necessary, to date, to isolate specific cell types from complex tissues. We have developed 'TaDa', a technique that enables cell-specific profiling without ...
ORGANISM(S): Drosophila melanogaster 
2013-06-21 | E-GEOD-46803 | biostudies-arrayexpress
ProteomicsMethylated-antibody affinity purification to improve proteomic identification of plant RNA polymerase Pol V complex and the interacting proteins
Affinity purification followed by enzymatic digestion and mass spectrometry has been widely utilized for the sensitive detection of interacting proteins and protein complexes in various organisms. In plants, the method is technically challenging due to the low abundance proteins, non-specific bindin...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-02-28 | PXD005422 | Pride
On-bead digestion Pol v complex Chemical cross-link Reductive methylation Ap-ms 
TranscriptomicsThe RNA Pol II Elongation Factor Ell3 Marks Enhancers in ES Cells and Primes Future Gene Activation
Enhancers play a central role in precisely regulating the spatiotemporal expression of developmentally regulated genes. The molecular mechanisms and factors required for controlling the inactive or poised enhancers and their function in future gene activation remain elusive. Here, we have identified...
ORGANISM(S): Mus musculus 
2012-12-30 | E-GEOD-38148 | biostudies-arrayexpress
TranscriptomicsCharacterization of small RNA-generating regions targeted by RNA polymerase V in Arabidopsis
In plants, heterochromatin is maintained by a small RNA-based gene silencing mechanism known as RNA-directed DNA methylation (RdDM). RdDM requires the non-redundant functions of two plant-specific DNA-dependent RNA polymerases (Pol) Pol IV and Pol V. Pol IV plays a major role in siRNA biogenesis, w...
ORGANISM(S): Arabidopsis thaliana 
2012-06-11 | E-GEOD-36424 | biostudies-arrayexpress
GenomicsHuman RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors.
RNA polymerase (Pol) III transcribes many noncoding RNAs (for example, transfer RNAs) important for translational capacity and other functions. We localized Pol III, alternative TFIIIB complexes (BRF1 or BRF2) and TFIIIC in HeLa cells to determine the Pol III transcriptome, define gene classes and r...
ORGANISM(S): Homo sapiens 
2010-04-14 | E-GEOD-20309 | biostudies-arrayexpress
GenomicsA conserved Pol II elongator SPT6L mediates Pol V transcription to regulate RNA-directed DNA methylation in Arabidopsis
A conserved Pol II elongator SPT6L mediates Pol V transcription to regulate RNA-directed DNA methylation in Arabidopsis
PRJNA977970 | ENA
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