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MetabolomicsAntioxidant Activity, Phytochemical Profiling by UPLC-QTOF-MS, and In Silico Targeting of the RNA-Binding Protein DRBD18 of Trypanosoma brucei by Bioactive Compounds from Tetradenia riparia and Tetracera poggei
Oxidative stress plays a key role in the pathogenesis of African trypanosomiasis, and plant-derived antioxidants represent a promising source of dual-action compounds capable of both scavenging reactive oxygen species and interfering with parasite survival. The present study investigates the antioxi...
2026-09-10 | MTBLS15597 | MetaboLights
TranscriptomicsGenome-wide study of nuclear poly(A) binding protein Nab2p depletion
Polyadenylation of mRNA is a key step in eu- karyotic gene expression. However, despite the ma- jor impact of poly(A) tails on mRNA metabolism, the precise roles of poly(A)-binding proteins (PABPs) in nuclear mRNA biogenesis remain elusive. Here, we demonstrate that rapid nuclear depletion of the S....
ORGANISM(S): Saccharomyces cerevisiae 
2015-08-13 | E-GEOD-65001 | biostudies-arrayexpress
TranscriptomicsThe Poly(C) binding protein Pcbp2, and its retrotransposed derivative Pcbp1, are independently essential to mouse development
RNA-binding proteins participate in a complex array of post-transcriptional controls essential to cell-type specification and somatic development. Despite their detailed biochemical characterizations, the degree to which each RNA-binding protein impacts on mammalian embryonic development remains in...
ORGANISM(S): Mus musculus 
2015-11-05 | E-GEOD-72491 | biostudies-arrayexpress
GenomicsPAPD5, a noncanonical poly(A) polymerase with an unusual RNA-binding motif [PAR-CLIP]
PAPD5 is one of the seven members of non-canonical poly(A) polymerases in human cells. There are previous reports about polyadenylation dependent degradation of pre-ribosomal RNAs and uridylation dependent degradation of histone mRNAs in vivo. In this study, we observed polyadenylation but not polyu...
ORGANISM(S): Homo sapiens 
2012-04-02 | E-GEOD-36987 | biostudies-arrayexpress
ProteomicsYBX3 is a versatile RNA-binding protein that controls amino acid import by regulating SLC mRNA abundance
Summary: Amino acids (AA) support protein synthesis, and thus cell growth and proliferation. Specialized transporters mediate AA exchange across membranes and their regulation is critical for AA homeostasis. Here, we report that the DNA- and RNA-binding protein YBX3 regulates the expression of AA t...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-11-06 | MSV000083108 | MassIVE
TranscriptomicsSaccharomyces cerevisiae 3' poly(A) site mapping
The use of alternative polyadenylation sites is common and affects the post-transcriptional fate of mRNA, including its stability, localization, and translation. Here we present a method for genome-wide and strand-specific mapping of poly(A) sites and quantification of RNA levels at unprecedented ef...
ORGANISM(S): Saccharomyces cerevisiae 
2013-01-10 | E-GEOD-40110 | biostudies-arrayexpress
TranscriptomicsPoly(A) tail length profiling of mRNAs from fission yeast
To obtain global data on polyadenylation of mRNAs, we fractionated the mRNAs according to their poly(A) tail length using a poly-U sepharose column followed by differential elution at five temperatures. Five mRNA fractions with distinct ranges of poly(A) tail length were then hybridized to microarr...
ORGANISM(S): Schizosaccharomyces pombe 
2007-05-16 | E-TABM-217 | biostudies-arrayexpress
TranscriptomicsIntegrative genome-wide analysis reveals HLP1, a novel RNA-binding protein? regulates plant flowering by targeted alternative polyadenylation
Alternative polyadenylation (APA) is a widespread mechanism for gene regulation and has been implicated in flowering, but the molecular basis governing the choice of a specific poly(A) site during the vegetative-to-reproductive growth transition remains unclear. Here we characterize HLP1, an hnRNP A...
ORGANISM(S): Arabidopsis thaliana 
2015-06-30 | E-GEOD-39051 | biostudies-arrayexpress
ProteomicsIdentification of the proteins that stably or transiently interact with the nuclear poly(A)-binding protein Pab2 in the fission yeast Schizosaccharomyces japonicus using the proximity-labeling method TurboID.
We looked for the proteins that associate with Pab2, a nuclear poly(A)-binding protein, in Schizosaccharomyces japonicus through the proximity-labeling technology TurboID followed by mass spectrometry. We revealed that Pab2 interacts with two putative RNA-binding proteins, Red5 and Rmn1, and a yeast...
ORGANISM(S): Schizosaccharomyces japonicus Schizosaccharomyces 
2025-03-13 | PXD053432 | Pride
Fission yeast Nuclear poly(a)-binding protein Schizosaccharomyces japonicus 
TranscriptomicsRNAcompete: a method for rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins
Metazoan genomes encode hundreds of RNA binding proteins (RBPs) but relatively few have well-defined RNA-binding preferences. Current techniques for determining RNA targets, including those involving in vitro selection and RNA co-immunoprecipitation, require significant time and labour investment. H...
ORGANISM(S): synthetic construct 
2010-05-18 | E-GEOD-15769 | biostudies-arrayexpress
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