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ProteomicsIdentification of proximal interactome of BioID2-tagged mitochondrial Top3α
To interrogate the role of Top3a in mtDNA replication progression, we studied the interaction of mitochondrial Top3a with known mitochondrial DNA maintenance proteins using proximity labelling proteomics. For this experiment, FlpIN-TRex 293 cell line expressing mitochondrial Top3a tagged with BioID2...
ORGANISM(S): Homo sapiens (Human) 
2022-07-22 | PXD035416 | Pride
Human Q exactive Mitochondria Bioid2 Top3α 
ProteomicsOptimising complexome profiling for systematic exploration of DNA-/RNA-protein complexes in human mitochondria
Here we aimed to improve native gel electrophoresis-based complexome profiling (CP) method for examination of mitochondrial (mt)DNA-/RNA-protein complexes. We compared the performance of Blue Native PAGE (BNE) and high resolution Clear Native PAGE (hrCNE). We found that hrCNE much better preserves t...
ORGANISM(S): Homo sapiens (Human) 
2023-08-11 | PXD040103 | Pride
Mtdna Mitochondria Mass spectrometry Dna-protein complex Native gel electrophoresis Rna-protein complex Complexome profiling 
ProteomicsIdentification of proximal interactome of c17orf80 C- and N-terminally tagged with BirA*
To validate the interaction of c17orf80 with mitochondrial nucleoid, we used proximity-labelling proteomics. We tagged c17orf80 with BirA* from either C- or N-terminus and expressed the fusion proteins in FlpIN-TRex 293 cell line. As controls for endogenous biotinylation, non-induced cells were used...
ORGANISM(S): Homo sapiens (Human) 
2023-07-11 | PXD037857 | Pride
C17orf80 Mitochondria Bioid 
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