To validate the interaction of c17orf80 with mitochondrial nucleoid, we used proximity-labelling proteomics. We tagged c17orf80 with BirA* from either C- or N-terminus and expressed the fusion proteins in FlpIN-TRex 293 cell line. As controls for endogenous biotinylation, non-induced cells were used...
The Crabtree effect, in which fermentative metabolism is preferred at the expense of respiration, is a hallmark of budding yeast’s glucose response and a model for the Warburg effect in human tumors. While the glucose-responsive transcriptional repressors Mig1p and Mig2p play well-characterized rol...
