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TranscriptomicsTranscription profiling by array of Legionella pneumophila ppGpp knockout and relA over expression mutants.
Effect of ppGpp on transcript level after RelA overexpression (90 min). The cells are constructed in our lab. They are knock-out mutants in the production of ppGpp(guanosine tetraphosphate) of Legionella, complemented with an over-expression vector containing the relA gene to express the RelA prot...
ORGANISM(S): Legionella pneumophila 
2010-04-14 | E-MTAB-211 | biostudies-arrayexpress
ProteomicsInteraction-based capture and identification of protein effectors of ppGpp, the master regulator of bacterial growth
The small nucleotide ppGpp is a conserved regulatory molecule in prokaryotes that tunes growth rate in response to nutrient availability. How ppGpp regulates growth remains poorly defined. In Escherichia coli, ppGpp directly binds RNA polymerase to modulate transcription, but it likely has other tar...
ORGANISM(S): Escherichia coli 
2018-10-04 | PXD010402 | Pride
E. coli Ppgpp Lc-ms/ms 
ProteomicsFine-tuning regulation of (p)ppGpp-driven outer membrane vesicle formation in Acinetobacter baumannii
While the roles of (p)ppGpp on cytosolic proteins are well established, its effects on membrane remodeling remain elusive. The translocation of signal recognition particle (SRP)-dependent proteins can be regulated by binding (p)ppGpp to two key GTPase components: FtsY, which interacts with SecYEG, a...
ORGANISM(S): Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377) 
2025-03-15 | PXD057710 | Pride
Membrane remodeling Membrane disruption Acinetobacter baumannii Outer membrane vesicle Stringent response (p)ppgpp 
ProteomicsE.coli (p) ppGpp regulation.E. coli, ppGpp, growth rate
These two projects are about the proteome of wild type E. coli K-12 NCM3722 strain (RE198LQ) and its rpoS-null strain (XA00994LQ). Both projects include replicates of two conditions, the normal conditions and (p)ppGpp overproduction conditions via RelA* OE.
ORGANISM(S): Escherichia coli O104:H4 str. 01-09591 
2023-12-20 | PXD042250 | Pride
Growth rate E. coli Ppgpp 
TranscriptomicsTranscription profiling by array of Legionella pneumophilaEfter RelA overexpression.
Effect of ppGpp on transcript level after RelA overexpression (45 min). The cells are constructed in our lab. They are knock-out mutants in the production of ppGpp(guanosine tetraphosphate) of Legionella, complemented with an over-expression vector containing the relA gene to express the RelA prot...
ORGANISM(S): Legionella pneumophila 
2010-04-13 | E-MTAB-222 | biostudies-arrayexpress
TranscriptomicsEffect of ppGpp on Salmonella Typhimurium gene expression
The expression profile of an S. Typhimurium mutant strain unable to synthesise ppGpp (relAspoT deletions) was compared to the wild-type strain. The effect of ppGpp on virulence gene expression was studied under 4 different growth conditions that induce virulence gene expression. Keywords: genetic mo...
ORGANISM(S): Salmonella typhimurium 
2010-06-11 | E-GEOD-4631 | biostudies-arrayexpress
Proteomics(p)ppGpp controls stringent factors by exploiting antagonistic allosteric coupling between catalytic domains
Escherichia coli RelA is a ribosomal factor with strong (p)ppGpp synthesis activity that is dramatically activated in the presence of deacylated tRNA in the ribosomal A-site. RelA is a unique enzyme in that it is directly positively regulated by its product, alarmone nucleotide (p)ppGpp. Using HDX-M...
ORGANISM(S): Escherichia coli 
2021-08-11 | PXD019953 | Pride
Escherichia coli / stringent response / rela / (p)ppgpp 
ProteomicsThe alarmone (p)ppGpp is part of the heat shock response of Bacillus subtilis
Here, B. subtilis was used as a model organism to investigate how cells respond and adapt to proteotoxic stress conditions. Our experiments suggested that the stringent response, caused by raised levels of the (p)ppGpp alarmone, plays a role during thermotolerance development and the heat shock resp...
ORGANISM(S): Bacillus subtilis GB03 
2020-03-09 | PXD015416 | Pride
Bacillus subtilis  heat stress response  stringent response  (p)ppgpp  alarmone  rel  spx 
TranscriptomicsMicroarray analysis of the effect of ppGpp, cdiAMP, and cdiGMP on Saccharomyces cerevisiae
Apart from the induction of host immune responses, any influence mediated by the presence of bacterial signalling molecules inside host cells on the latter’s physiology remains poorly defined. To determine the effects of the presence of ppGpp, cdiAMP and cdiGMP in the cytosol on the functioning of...
ORGANISM(S): Saccharomyces cerevisiae 
2017-07-26 | E-MTAB-5128 | biostudies-arrayexpress
GenomicsEffect of ppGpp on Salmonella Typhimurium gene expression
Effect of ppGpp on Salmonella Typhimurium gene expression
PRJNA95531 | ENA
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